Invitrogen™ AccuPrime™ Taq DNA Polymerase System

Suitable for wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications

Includes: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl₂, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.

Catalog Number	Mfr. No.	No. of Reactions
12-339-016	Invitrogen™ 12339016	200 Reactions
12-339-024	Invitrogen™ 12339024	1000 Reactions

Description

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases. Platinumanti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, providing optimal performance between 55°C and 65°C.

• Specificity: Control mispriming at every cycle to get single-band PCR products
• Ease of use: minimize reaction optimization and primer set redesign
• Convenience: conveniently assemble reactions at room temperature

• AccuPrime buffers: 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg⁺⁺, and deoxyribonucleotide triphosphates at concentrations
• Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
• For small genomic DNA amplicons (≤200bp), cDNA, or plasmids

Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, Multiplex PCR, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Detection Method: Primer-Probe
• GC-Rich PCR Performance: Low
• Polymerase: AccuPrime Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase System
• For Use With (Application): Cloning
• Fidelity (vs. Taq): 2 X
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• Format: Tube
• PCR Method: 1-step RT-qPCR, Multiplex qPCR
• Reaction Speed: Standard
• Product Line: AccuPrime™
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Concentration: 10X
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.