Invitrogen™ AccuPrime™ Taq DNA Polymerase System

The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Supplier:  Invitrogen™ 12339024

Catalog No. 12-339-024

Includes: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl₂, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.

Description

The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum™ anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

Advantages of AccuPrime™ Taq DNA Polymerase:
• Specificity—control mispriming at every cycle to get single-band PCR products
• Ease of use—minimize reaction optimization and primer set redesign
• Convenience—conveniently assemble reactions at room temperature

AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg⁺⁺, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).

Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: • AccuPrime Taq DNA Polymerase (500 μL, at 2 U/μL)
  • 10X AccuPrime PCR Buffer I (2 x 1.25 mL)
  • 10X AccuPrime PCR Buffer II (2 x 1.25 mL)
  • 50 mM Magnesium Chloride (2 x 1.25 mL)
  
  Store at -20°C.
• GC-Rich PCR Performance: Low
• Polymerase: AccuPrime Taq DNA Polymerase
• Product Type: DNA Polymerase System
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 2X
• No. of Reactions: 1000 Reactions
• Reaction Format: Separate Components

• Detection Method: Primer-probe
• PCR Method: 1-step RT-qPCR, Multiplex qPCR
• Reaction Speed: Standard
• Shipping Condition: Wet or Dry Ice
• Concentration: 10X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.