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Invitrogen™ AccuPrime™ Taq DNA Polymerase System
The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
Supplier: Invitrogen™ 12339016
Includes: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
Description
The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum™ anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.
Advantages of AccuPrime™ Taq DNA Polymerase:
• Specificity—control mispriming at every cycle to get single-band PCR products
• Ease of use—minimize reaction optimization and primer set redesign
• Convenience—conveniently assemble reactions at room temperature
AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications.
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifications
• AccuPrime Taq DNA Polymerase (100 μL, at 2 U/μL) • 10X AccuPrime PCR Buffer I (500 μL) • 10X AccuPrime PCR Buffer II (500 μL) • 50 mM Magnesium Chloride (500 μL) Store at -20°C. |
|
Low | |
AccuPrime Taq DNA Polymerase | |
DNA Polymerase System | |
Hot-start PCR | |
2X | |
200 Reactions | |
Separate Components |
Primer-probe | |
1-step RT-qPCR, Multiplex qPCR | |
Standard | |
Wet or Dry Ice | |
10X | |
Built-In Hot Start | |
3'-A | |
5 kb or less |
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For Research Use Only. Not for use in diagnostic procedures.