Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

Includes: Contents: 5 x 200μL of AmpliTaq DNA Polymerase (5U/μL); 4 x 1.5mL of GeneAmp 10X PCR Buffer II (500mM KCl, 100mM Tris-HCl pH 8.3); 4 x 1.5mL of 25mM MgCl₂.

Catalog Number	Mfr. No.	Quantity
N8080160	Applied Biosystems™ N8080160	250 Units
N8080171	Applied Biosystems™ N8080171	1000 Units
N8080152	Applied Biosystems™ N8080152	3000 Units
N8080155	Applied Biosystems™ N8080155	5000 Units
N8080185	Applied Biosystems™ N8080185	25,000 Units

Description

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.

Features of this enzyme:

• AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
• Its thermal activity profile makes it reliable for PCR applications
• It is QC-tested to guarantee reproducible results

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C to 75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl₂ Solution.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

Order Info

Shipping Condition: Dry ice

Specifications

• Format: Stand-alone enzyme
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• Polymerase: AmpliTaq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: No
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.