Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

Supplier:  Applied Biosystems™ N8080160

Catalog No. N8080160

Includes: Contains 1000 units of AmpliTaq DNA Polymerase, Buffer II, and MgCl₂ Solution.

Description

AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.

AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl₂ Solution.

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.

Features

• The most thoroughly characterized enzyme available for the PCR process
• Excellent thermal activity profile makes it good for PCR applications
• QC-tested to guarantee reproducible results

Notes

• For superior PCR performance, we recommend DreamTaq DNA Polymerase.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: • AmpliTaq DNA Polymerase (5 U/μL), 50 μL
  • GeneAmp 10X PCR Buffer, 1.5 mL
  
  Store at -20°C.
• Polymerase: AmpliTaq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 250 Units
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 200 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Format: Stand-alone enzyme
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: No
• Overhang: 3'-A
• Size (Final Product): 5 kb or less
• Includes: Buffer I

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.