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Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
Supplier: Applied Biosystems™ N8080155
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Includes: Contents: 5 x 200μL of AmpliTaq DNA Polymerase (5U/μL); 4 x 1.5mL of GeneAmp 10X PCR Buffer II (500mM KCl, 100mM Tris-HCl pH 8.3); 4 x 1.5mL of 25mM MgCl2.
Description
AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot.
Features of this enzyme:
- AmpliTaq DNA Polymerase is the most thoroughly characterized enzyme available for PCR, a testimony to its overall utility and efficacy
- Its thermal activity profile makes it reliable for PCR applications
- It is QC-tested to guarantee reproducible results
Reliable and robust PCR
The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55°C to 75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq DNA Polymerase is supplied with GeneAmp10X PCR Buffer I. It is also available with GeneAmp 10X PCR Buffer II and MgCl2 Solution.
For superior PCR performance, we recommend DreamTaq DNA Polymerase.
Order Info
Shipping Condition: Dry ice
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Specifications
• AmpliTaq DNA Polymerase (5 U/μL), 5 x 200 μL • GeneAmp 10X PCR Buffer, 20 x 1.5 mL Store at -20°C. |
|
AmpliTaq DNA Polymerase | |
5' - 3' | |
5000 Units | |
Standard PCR | |
1X | |
4000 Reactions | |
Separate Components | |
DNA |
Stand-alone enzyme | |
Standard | |
DNA Polymerase | |
Dry Ice | |
5 U/μL | |
No | |
3'-A | |
5 kb or less |
Safety and Handling
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For Research Use Only. Not for use in diagnostic procedures.