Reverse Transcription Products
Reverse Transcription Products
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Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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GC-Rich PCR Performance | High |
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Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima H Minus |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 30 to 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ MultiScribe™ Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript II |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 50 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Kit |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Verso cDNA Synthesis Kit
The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.
Shipping Condition | Wet or Dry Ice |
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Format | Kit |
Reverse Transcriptase | Verso |
Size (Final Product) | Up to 11 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix
Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima H Minus |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Format | Master Mix |
Size (Final Product) | Up to 20 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
For Use With (Application) | RT-PCR |
Format | Kit |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR
The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | Maxima |
Final Product Type | First-Strand cDNA |
For Use With (Application) | Real Time PCR (qPCR) |
Format | Kit |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Format | Kit |
Reverse Transcriptase | Maxima H Minus |
Size (Final Product) | Up to 20 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C to 55°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
Concentration | 200 U/μL |
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