Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

$672.65 - $5340.00


Detection Method qPCR
Format Tube
GC-Rich PCR Performance High
PCR Method 2-step RT-qPCR
Reaction Speed 10 min.
View More Specs

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
View Documents
50 Reactions
Each for $672.65
Add to cart
View Documents
500 Reactions
Each for $5,340.00
Add to cart


SuperScript™ IV VILO™ (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Inclusion of ezDNase, further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. This master mix is also available without ezDNase, which can be purchased separately.

Features of SuperScript IV VILO Master Mix include:
  • Super-fast RT reaction in just 10 minutes and gDNA removal in 2 minutes
  • Super-high yields—lower Ct values by more than 2 cycles ahead of all other reverse transcription reagents
  • Super-convenient one-tube cDNA reaction master mix for two-step RT-qPCR
  • Super-efficient reaction even with low template amounts and suboptimal purity samples
SSIV VILO master mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript™ IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

For even better performance, some SuperScript IV VILO Master Mix products include ezDNase (also available as a separate purchase) for genomic DNA removal to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix gives you more cDNA in less time with less pipeting, less variation, less reaction inhibition, less gDNA interference, and with less sample than all the other cDNA synthesis kits or master mixes for RT-qPCR.

Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity.

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product.

For Research Use Only. Not for use in diagnostic procedures.


10 min.
First-Strand cDNA
Reverse Transcription
2-step RT-qPCR
Reverse Transcription
SuperScript IV
Dry Ice
Master Mix
Up to 10 kb
Product Certifications
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit