Invitrogen™ MultiScribe™ Reverse Transcriptase

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.

Supplier:  Invitrogen™ 4311235

Catalog No. 43-112-35

Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL).

Description

MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays. MultiScribe Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. This recombinant RNA–dependent, DNA polymerase uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand.

Features of MultiScribe Reverse Transcriptase include:
• Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay setup
• Individual component design provides versatility in assay setup
• 5,000 U is supplied in 100 μL vials (50 U/μL)
• Optimal reaction temperature: 42–52°C

Applications
• PCR
• RT-PCR
• Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage:

  • MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
  
  Store at –20°C.
  
  

• Technique: Reverse Transcription
• Reverse Transcriptase: MultiScribe
• Shipping Condition: Dry Ice
• Concentration: 50 U/μL
• Reaction Format: Separate components
• Size (Final Product): Up to 7 kb

• Format: Stand-alone Enzyme
• Optimal Reaction Temperature: 37°C
• Ribonuclease H Activity: Reduced
• For Use With (Application): RT-PCR
• Final Product Type: First-Strand cDNA
• Reagent Type: Reverse Transcription
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.