Test
Use for the rapid purification of DNA >10 base pairs in length in a wide range of applications. Cytiva Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
---|---|
Product Type | NAP-10 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Use for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides. Cytiva Life Sciences™ illustra™ MicroSpin™ S-400 HR Columns use spin-column chromatography for quick results.
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
---|---|
Product Type | NAP-5 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Designed to allow the rapid purification of DNA for use in a wide range of applications using typical microfuge equipment. Cytiva illustra™ Microspin G-50 Columns will not remove or denature enzyme.
Includes | Sephadex™ G-50 DNA grade |
---|---|
Product Type | Column |
For Use With (Application) | Desalting, Buffer exchange, removal of dye terminators from cycle sequencing reactions, removal of labeled, Nucleotides in 25 to 50μL from DNA labeling reactions |
For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50μL. Cytiva Life Sciences™ ProbeQuant™ G-50 Micro Columns use spin-column chromatography.
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
---|---|
Product Type | NAP-25 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Includes | Spin columns prepacked with a novel Silica membrane, Suspension, Lysis and Neutralization Buffers containing chaotropic salts, Wash and Elution Buffers, microcentrifuge collection tubes, full protocol booklet, Detachable, quick reference protocol card |
---|---|
Description | Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications. |
Product Type | illustra plasmidPrep Mini Spin Kit |
Purity | 1.7 |
Yield | 6-15 μg |
For Use With (Application) | Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma |
Test Time | Approx. 9 min |
Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Includes | 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA |
---|---|
Content And Storage | The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification |
Description | illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. |
For Use With (Application) | Sequencing Template Amplification Kits and Reagents |
Test Time | 4-18 h depending on starting material |
Enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs
Description | Enzymatic PCR clean up technology with Exonuclease I and Shrimp Alkaline Phosphatase to remove unincorporated primers and dNTPs. For maximum flexibility comes in two separate tubes. |
---|---|
Product Type | illustra ExoProStar S |
For Use With (Application) | PCR and Sequence Reaction;Removes unincorporated primers and dNTPs; Improves digestion efficiency; No loss of PCR product |
Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA specifically from large constructs (e.g., fosmids and BACs) sufficient for DNA sequencing
illustra Ready-To-Go GenomiPhi HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60++g yield from nanogram amounts of DNA sample.
Content And Storage | Store the kit at ambient temperature.Once opened, completely reseal the pouch containingthe Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended.Store recons |
---|---|
Description | illustra Ready-To-Go GenomiPhi HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample. |
Size | > 10 kb |
For Use With (Application) | Genomic DNA Amplification |
Test Time | 4 h |
Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications
For rapid purification of labeled single-stranded or double-stranded DNA fragments ≥50 bases in length in a volume of 25 to 100μL using spin-column chromatography
Purify and concentrate PCR products or DNA fragments ranging in size from 50 bp to 10 kb. Cytiva Life Sciences™ illustra™ GFX™ PCR DNA and Gel Band Purification Kit can be used to purify DNA from reaction volumes up to 100μL or agarose gel slices of up to 900mg.
Includes | GFX™ columns, collection tubes, color-coded bottles of capture Buffer, Wash Buffer, two Elution Buffers |
---|---|
Description | Designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. |
Product Type | illustra GFX PCR DNA and Gel Band Purification Kit |
Formulation | Tris HCl and sterile Water |
Sample Type | Agarose Gel Slices,DNA Solutions,Enzyme Reactions,PCR Mixtures |
Sample Volume | 50 bp-10 kb |
Yield | PCR 10 μl elution volume-65%, PCR 50 μl elution volume-82%, 300 mg agarose 10 μl elution volume-57%, 300 mg agarose 50 μl elution volume-91% |
For Use With (Application) | Isolation and concentration of DNA fragments (50bp to 10kb) from PCR mixtures, DNA-containing Agarose Gel bands, Enzyme-based DNA modifications and restriction digestions |