Cytiva TempliPhi™ 2000 Reaction Kit

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications

Supplier:  Cytiva 28964286

Catalog No. 45-003-001

Includes: TempliPhi Premix, Denature buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)

Description

• Simple protocol reduces time, labor and consumables needed for template preparation, and workflow enables easy automation
• Generate microgram quantities of template DNA from picogram amounts of starting material
• Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks or purified circular (plasmid or M13) DNA
• Use amplified DNA directly for cycle sequencing without purification
• Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h

Specifications

• Content And Storage: Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C.Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior
• For Use With (Application): High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
• No. of Reactions: 2000
• Test Time: 16-18 h

• Description: illustra TempliPhi 2000 Reaction Kit
• Includes: TempliPhi™ premix, denature Buffer, Enzyme mix and positive control pUC19 DNA
• Product Type: illustra TempliPhi 2000 Reaction Kit