Cytiva TempliPhi™ 100/500 Amplification Kit

Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.

Includes: Sample buffer, Reaction buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)

Catalog Number	Mfr. No.	No. of Reactions
45-002-262	Cytiva 25640010	100
45-002-265	Cytiva 25640050	500

Description

• Generate microgram quantities of template DNA from picogram amounts of starting material
• Completed reactions contain uniform quantities of DNA irrespective of input amount, allowing for use directly in sequencing reactions without quantification
• Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA without the need for purification
• Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation
• Contain separate, unmixed components for enhanced stability

Specifications

• Content And Storage: The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification
• For Use With (Application): Sequencing Template Amplification Kits and Reagents
• Test Time: 4-18 h depending on starting material

• Description: illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
• Includes: 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA