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Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.
CAS: 9007-49-2 Molecular Formula: C15H31N3O13P2 Molecular Weight (g/mol): 0.00 MDL Number: MFCD00130921 InChI Key: AWBASQCACWFTGD-UHFFFAOYNA-N IUPAC Name: Deoxyribonucleic acid SMILES: *
CAS | 9007-49-2 |
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Molecular Weight (g/mol) | 0.00 |
MDL Number | MFCD00130921 |
SMILES | * |
IUPAC Name | Deoxyribonucleic acid |
InChI Key | AWBASQCACWFTGD-UHFFFAOYNA-N |
Molecular Formula | C15H31N3O13P2 |
Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction
The complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute or less, room temperature reaction
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Promoter | T7, SP6 |
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Content And Storage | This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TA Cloning |
Bacterial or Yeast Strain | Not Included |
For Use With (Application) | PCR Cloning |
Vector | pCRII |
Product Line | TA Cloning™ |
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.