Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ GeneArt™ Gibson Assembly EX Cloning Kit, electrocompetent cells

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

Supplier:  Invitrogen™ A46634

Encompass_Preferred

Catalog No. A46634


Explore available promotions
Add to Cart

Description

Description

The GeneArt Gibson Assembly EX Cloning Kit enables DNA assembly via a technique that allows up to 15 overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction. DNA fragments of different lengths are uniformly assembled using complementary overlaps between fragments. The inherent flexibility and success of this approach is suitable for small and large DNA constructs, includes both single and multiple inserts, and has been used to build entire genomes. The resulting products can be used for a variety of downstream applications, including transformation, PCR, and rolling circle amplification (RCA). The GeneArt Gibson Assembly EX Cloning Kit, electrocompetent cells, is a complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Features of the GeneArt Gibson Assembly EX Cloning Kit include:

  • Simple—seamlessly assemble and clone up to 15 DNA fragments in a single reaction
  • Efficient—robust efficiency provides successful clones for both simple and challenging constructs
  • Flexible—design guidelines allow assembly into any vector of your choice
  • Convenient—available as master mix kits and cloning kits complete with chemical or electrocompetent cells
  • Trusted—over 4000 citations in the scientific literature highlighting great success

Seamless assembly of up to 15 fragments

GeneArt Gibson Assembly EX cloning is a robust, single-tube, two-step process whereby up to 15 inserts and vector are combined in a proprietary enzymatic mix in order to assemble DNA fragments with shared terminal end homology without leaving any extra sequences or scars behind (seamless). After this dually optimized reaction is complete, a portion of the assembly reaction is then transformed into chemically competent or electrocompetent cells, yielding clones ready for analysis the next day. The required 20- to 40-base pair end homology is designed into the de novo fragment for synthesis or can be easily engineered by PCR amplification with custom DNA oligos. This special enzyme mix creates a seamless and covalently bound DNA construct providing high efficiency.

Robust method for maximum efficiency

Due to the covalently bound final product, the GeneArt Gibson Assembly EX method allows the utilization of chemically competent cells or electrocompetent cells for the highest transformation efficiency, especially important for challenging constructs. There is no need for restriction enzymes, ligation, or recombination sites, and the method provides a perfectly seamless construct without unwanted extra bases.

Provides great versatility

The GeneArt Gibson Assembly EX method provides versatility, can streamline many techniques through the rapid combination, addition, deletion, or exchange of DNA segments, and eliminates the need to subclone, saving time and effort in the cloning workflow.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

ElectroMAX DH10B
Seamless cloning
Positive Control
Seamless Cloning & Genetic Assembly
Up to 15 fragments
Cloning Kit
DNA Assembly
Electrocompetent E. coli
A complete kit that includes master mix, positive control, water, and ElectroMAX DH10B electrocompetent E. coli.

Kit
10 Reactions
GENEART™
Dry Ice
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

For Research Use Only. Not for use in diagnostic procedures.