Real Time PCR Master Mixes
![](https://assets.fishersci.com/TFS-Assets/CCG/product-images/real-time-pcr-master-mixes-header-imag.jpg-250.jpg)
Real Time PCR Master Mixes
- (6)
- (3)
- (3)
- (230)
- (98)
- (20)
- (32)
- (2)
- (4)
- (44)
- (34)
- (30)
- (2)
- (1)
- (39)
- (84)
- (137)
- (4)
- (137)
- (118)
- (1)
- (24)
- (13)
- (5)
- (4)
- (28)
- (13)
- (223)
- (193)
- (1)
- (1)
Filtered Search Results
![](/catalog/search/static/resources/images/icons/back_to_top_icon.png)
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Dry Ice |
---|---|
Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Product Line | Applied Biosystems™ |
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix (No ROX)
TaqPath 1-Step Multiplex Master Mix (No ROX) is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Dry Ice |
---|---|
Format | Tube |
For Use With (Equipment) | QuantStudio™ 6 Pro or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, ViiA™ 7, 7500, 7500 Fast, 7500 Fast Dx, or use a compatible real-time PCR instrument from another supplier |
Sample Type | DNA, RNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
GC-Rich PCR Performance | High |
---|---|
Purity or Quality Grade | UP (Ultra Pure) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR even in the presence of challenging PCR inhibitors.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Dry Ice |
---|---|
Reverse Transcriptase | M-MLV |
Form | Liquid |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Format | Tube |
Product Type | 1-Step Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | Total RNA, Viral DNA, Viral RNA, mRNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqMan™ |
Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
GC-Rich PCR Performance | High |
---|---|
Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,miRNA analysis,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | TaqMan™ |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Polymerase | Fast DNA Polymerase |
---|---|
Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Wet Ice |
---|---|
Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUP™, SYBR™ |
Detection Method | SYBR |
Fisherbrand™ SYBR™ Green qPCR Master Mix
Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Content And Storage | SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark |
Product Type | Master Mix |
Form | Frozen |
Reaction Speed | Fast or Standard |
Label or Dye | SYBR Green,FAM |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR) |
PCR Method | qPCR,2-step RT-qPCR,RT-qPCR |
Detection Method | SYBR |
Applied Biosystems™ TaqPath™ ProAmp™ Master Mix
TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Wet Ice |
---|---|
Polymerase | Taq DNA Polymerase |
Content And Storage | Store at 2°C to 8°C in the dark. |
Product Type | qPCR Master Mix |
For Use With (Equipment) | 7500 Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, StepOne™ System |
Reaction Speed | Fast or Standard |
For Use With (Application) | Genotyping |
PCR Method | qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ Absolute Q™ DNA Digital PCR Master Mix (5X)
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Applied Biosystems™ TaqPath™ 1-Step Multiplex Master Mix
TaqPath 1-Step Multiplex Master Mix is a fast, single-tube, 4X RT-qPCR mix that provides for sensitive, reproducible detection of up to four different RNA/DNA targets in a single multiplex reaction.
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Dry Ice |
---|---|
Polymerase | Fast DNA Polymerase |
Format | Tube |
Product Type | qPCR Master Mix |
For Use With (Equipment) | QuantStudio™ 6 Pro Real-Time PCR System or QuantStudio™ 7 Pro, QuantStudio™ 3 or 5, QuantStudio™ 6/QuantStudio™ 7 Flex, QuantStudio™ 12K Flex, QuantStudio™ Dx, ViiA™ 7, 7500 , 7500 Fast, 7500 Fast Dx |
Sample Type | DNA, RNA |
Reaction Speed | Fast or Standard |
Concentration | 4X |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox
TaqPath™ BactoPure™ Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors. Does not contain a passive reference, recommended for use with assays utilizing JUN dye or higher order multiplexing (> three targets).
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Content And Storage | Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes: • Hot-start AmpliTaq Fast DNA Polymerase • Heat-labile uracil-DNA glycosylase (UNG) • dNTPs (with dUTP) • Optimized buffer Store at 2–8°C. |
---|---|
Passive Reference Dye | None |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™ 12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
For Use With (Application) | Microbial Detection |
Product Line | TaqPath™ |
Applied Biosystems™ TaqPath™ qPCR Master Mix, CG
TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Content And Storage | Store at 2°C to 8°C in the dark. |
---|---|
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,Virus Detection |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | Fast DNA Polymerase |
Format | Bottle |
Product Type | qPCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | QuantStudio™ Dx, 7500 Fast Dx |
Sample Type | cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Product Line | TaqPath™ |