Applied Biosystems™ TaqPath™ qPCR Master Mix, CG

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Catalog Number	Mfr. No.	Quantity
A15298	Applied Biosystems™ A15298	1 x 50 mL
A16248	Applied Biosystems™ A16248	10 x 5 mL
A16247	Applied Biosystems™ A16247	5 x 5 mL

Description

The master mix delivers confident results in both single and duplex reactions, even in the presence of inhibitors commonly found in clinical samples. All lots are functionally tested to help ensure lot-to-lot reproducibility for Ct consistency and dynamic range across a wide variety of assays. This General Purpose Reagent is labeled ‘For Laboratory Use’ and manufactured in an ISO 13485-certified and FDA-registered facility that adheres to cGMP principles. With stringent quality and premier performance, TaqPath qPCR Master Mix is a superior choice for your diagnostic testing or development needs.

Features of the TaqPath qPCR Master Mix include:
• Efficient and linear detection of up to eight logs with gene expression or miRNA assays
• Enables reliable detection of low-copy templates with reproducible CT results
• Robust multiplexing performance with exogenous or endogenous targets
• Manufactured with stringent production and process controls to help ensure lot-to-lot consistency
• Compliance Package containing most frequently requested compliance documents, available below

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Validated with a breadth of workflows
TaqPath qPCR Master Mix, CG, has been validated to provide high specificity and dynamic range for use in multiple real-time PCR applications. The formulation can be run in either fast or standard cycling conditions on a wide variety of qPCR platforms. The figure below demonstrates the excellent PCR linearity over an 8-log input range of template with both gene expression and miRNA assays.

In addition, TaqPath qPCR Master Mix, CG, has been engineered to retain consistent performance in preassembled reactions for up to 48 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. TaqPath qPCR Master Mix has been benchmarked against similar competitor master mixes and demonstrates equivalent or better sensitivity and dynamic range across a variety of targets.

Reproducible sensitive detection
We understand the importance of reliably detecting low-copy targets for your test quality and data interpretation. TaqPath qPCR Master Mix, CG, helps generate significant and reproducible CT values for ≤10 copy detection. The figure below shows the consistent CT results obtained from three unique lots when detecting 10-copy inputs. This lot-to-lot CT consistency is preserved across multiple assays with different attributes and expression levels to maximize confidence in your results.

Optimized for multiplexing
Simultaneous amplification of multiple assays can be beneficial not only as a control to normalize and detect anomalies in experiments, but also to improve efficiency and cost-savings for labs. TaqPath qPCR Master Mix has been optimized with enzymes and component concentrations to facilitate multiplexing while preserving specificity, and it is validated for duplex performance in each manufacturing lot.

Inhibitor tolerant
Unlike other master mixes on the market, TaqPath qPCR Master Mix’s unique proprietary formulation allows robust performance even in the presence of substances that can normally inhibit PCR, such as heparin, hematin, or EDTA, increasing your confidence when working with a variety of complex clinical samples. TaqPath master mixes demonstrate increased tolerance to inhibitors compared to competitor mixes.

Compliance Package
In an effort to efficiently support your compliance needs, frequently requested compliance documents are available here:
• Statement of Quality relating to the current good manufacturing practices (cGMP) to which TaqPath master mixes adhere
• Detailed lot-specific Certificate of Analysis (COA) (see below in Documents & Downloads section)
• Supplier Evaluation Support
   -- Completed Self Assessment
   -- ISO certificates by manufacturing site
• For EU Reach compliance-related inquiries, email 'LSGChemicalCompliance@thermofisher.com'
• BSE/TSE Statement
• Change Control and Notification Registration

Not finding the compliance document you’re looking for? Technical Support is here to help.

Specifications

• Content And Storage: Store at 2°C to 8°C in the dark.
• Form: Liquid
• PCR Method: qPCR
• Reaction Speed: Fast or Standard
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: qPCR Master Mix
• Concentration: 2X
• Sample Type: cDNA, DNA

• Detection Method: Primer-probe
• Format: Bottle
• Polymerase: Fast DNA Polymerase
• For Use With (Equipment): QuantStudio™ Dx, 7500 Fast Dx
• Product Line: TaqPath™
• For Use With (Application): Gene Expression, Virus Detection
• Hot Start: Built-In Hot Start