Western Blot Substrates and Substrate Kits

Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1 – 30 of 57 results
1

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world’s first commercially available chemiluminescent detection reagents and still one of the most widely used.

EDGE
Content And Storage 2°C to 8°C
Sensitivity ~10 pg
Product Type ECL Western Blotting Detection Reagent
For Use With (Application) Western Blot
2

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

EDGE
Substrate Western Chemiluminescent HRP
Content And Storage 2°C to 8°C
Shelf Life 9 Months
For Use With (Application) Dot/Slot Blot
3

MilliporeSigma™ Luminata Crescendo ELISA HRP Substrate

Used on immunoblots as chemiluminescent substrate

Content And Storage 2°C to 8°C
4

MilliporeSigma™ Calbiochem™ TMB, Insoluble

Peroxidase substrate which produces stable, insoluble, aquamarine blue precipitate at reaction site.

5

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Promotions are available
Content And Storage 2°C to 8°C
Sensitivity ~1 pg
Product Type ECL Prime Western Blotting Detection Reagent
For Use With (Application) Sensitive Quantitative or Confirmatory Western Blot
6

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

7

Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

8

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Substrate Horseradish Peroxidase
9

Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

10

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Substrate Horseradish Peroxidase
Form Liquid
Product Type Substrate
Signal Duration 6 to 8 hr.
Product Line SuperSignal™
Detection Method Chemiluminescent
11

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

12

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

13

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

14

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal™
Detection Method Chemiluminescent
15

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

ENCOMPASS
16

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.

17

MilliporeSigma™ Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

For Use With (Application) Dot/Slot Blot
18

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels.

Promotions are available
19

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

20

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Content And Storage 2°C to 8°C
Kit Contents Detection reagent 1 and 2
Product Type ECL 2 Western Blotting Substrate
21

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Shipping Condition Wet Ice
Substrate Horseradish Peroxidase
Sensitivity Low-femtogram to high-attogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal™
Detection Method Chemiluminescent
22

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

23

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

ENCOMPASS
24

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

25

Thermo Scientific™ NBT Substrate Powder (nitro-blue tetrazolium chloride)

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

26

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Substrate Horseradish Peroxidase
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form Liquid
Product Type Substrate
Signal Duration 1 to 2 hr.
Product Line Pierce™
Detection Method Chemiluminescent
27

Thermo Scientific™ BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine)

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate solutions for chromogenic detection of alkaline phosphatase probes.

28

MilliporeSigma™ Immobilon™ Block Noise Canceling Reagents

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Content And Storage 20°C to 25°C
Sufficient For Nineteen 8 x 7cm miniblots
Quantity 500 mL
29

R&D Systems™ VisULite MAX ECL Western Blotting Substrate

Ready-to-use ultra high sensitivity Enhanced Chemiluminescent (ECL) substrate.

30

Novus Biologicals™ LumiBlue (TM) ECL Extended

Content And Storage Store at 4°C
For Use With (Application) Western Blot