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Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
Recommended HRP substrate for fluorescence imagers
$156.60 - $1000.00
Specifications
Product Type | ECL Western Blot Substrate |
---|---|
Kit Contents | Detection reagent 1 and 2 |
Content And Storage | 2°C to 8°C |
Includes: Detection Reagent 1 and 2
Catalog Number | Mfr. No. | Quantity | Sufficient For | Price | Quantity & Availability | ||||
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Catalog Number | Mfr. No. | Quantity | Sufficient For | Price | Quantity & Availability | ||||
PI80196
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Thermo Scientific™
80196 |
100 mL | 10 Mini-gel blots, 1000cm2 of membrane |
Each for $505.50
|
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PI80196X3
|
Thermo Scientific™
80196X3 |
3 x 100 mL | 30 Mini-gel blots, 3000cm2 of membrane |
Each for $1,000.00
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PI80197
|
Thermo Scientific™
80197 |
25 mL | 250cm2 of membrane |
Each for $156.60
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Description
Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.
Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.
Highlights:
- High sensitivity — detect targets down to the low-picogram level
- Long signal duration — sustained light output for as long as five hours
- More imaging options — X-ray, CCD or laser-based imagers
- More affordable — high quality and performance at a lower price than other suppliers' similar substrates
Specifications
ECL Western Blot Substrate | |
Detection reagent 1 and 2 | |
2°C to 8°C |
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