This high performance thin layer chromatography (HPTLC) plate comprises a C18 Silica layer. The Nano-Silica 60 has a surface area of 500 m2/g, mean pore size of 60A, specific pore volume of 0.75 ml/g, particle size of 2-10um, and pH stability of 2-10. The surface has been 50% treated with an octadecyl silane so that it is a reversed phase material. This analytical hard layer is bound to a glass support. It has a thickness of 200um and dimensions of 10x10cm. There are 25 plates per pack. Applications include: Alkaloids, amino acids, preservatives, optical brighteners, barbiturates, polycyclic aromatic hydrocarbons (PAH), drugs, peptides, flavonoids, phenols, indole derivatives, steroids