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Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

Supplier:  Cytiva 17004101

Catalog No. 45-000-015

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  • For purposes of determining maximum flowrate, beads behave as rigid spheres according to Darcy's Law
  • Working pH range 2 to 10
  • Particle size range for wet beads 20 to 80μm
  • Bed volume in distilled water 9 to 11mL/g
  • Fractionation ranges: 5 x 102 to 1 x 104 for dextrans, 1.5 x 103 to 3 x 104 for globular proteins



4 to 30°C
< Darcy´s Law
9 to 11mg/g
Solid Bead
Purification of DNA from small molecules by Gel filtration (higher efficiency with shorter diffusion distances)
2 to 10
20 to 80 μm in wet form, 20 to 50 μm in dry form
Sephadex G-50 Superfine, 100 g
3.93 in.
min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.
Cross-linked Dextran


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