Cytiva Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Catalog Number	Mfr. No.	Flow Rate	Particle Size	Quantity
45-002-049	Cytiva 17003202	>47 cm/hr.	17 to 132 μm in wet form, >20 μm in dry form	500g
45-002-045	Cytiva 17003402	>480 cm/hr.	87 to 510 μm in wet form, >100 μm in dry form	500g
45-002-040	Cytiva 17003101	>11 cm/hr.	15 to 88 μm in wet form, >20 μm in dry form	100g
45-002-041	Cytiva 17003201	>47 cm/hr.	17 to 132 μm in wet form, >20 μm in dry form	100g
45-002-046	Cytiva 17003401	>480 cm/hr.	87 to 510 μm in wet form, >100 μm in dry form	100g
45-002-047	Cytiva 17003302	>100 cm/hr.	38 to 235 μm in wet form, >50 μm in dry form	500g
45-002-048	Cytiva 17003301	>100 cm/hr.	38 to 235 μm in wet form, >50 μm in dry form	100g

Description

Established medium for desalting and buffer exchange

 

• Gel filtration medium
• Available in coarse, medium, fine and superfine grades
• All grades satisfy process-scale chromatography requirements
• Available in lab packs or prepacked columns

 

Specifications:

• Working pH range: 2 to 13
• Bed volume (in distilled water): 4 to 6mL/g
• Fractionation range dextrans/globular proteins: 1 × 10² to 5 × 10³ / 1 × 10³ to 5 × 10³ M_r
• Max. flowrate/operating pressure: the beads behave as rigid spheres obeying Darcy's Law

 

Specifications

• Bed Volume: 4 to 6mL/g in Distilled Water
• Format: Solid Spherical Bead
• For Use With (Application): Desalting and Buffer exchange and removal free label in industrial applications
• Height (Metric): 30cm
• Support Type: Cross-linked Dextran

• Product Type: Sephadex G-25 Media
• Diameter (Metric) Inner: 2.6cm
• Height (English): 11.81 in.
• pH Range: 2 to 13
• Content And Storage: 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH