Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Supplier:  Thermo Scientific™ F531L

Catalog No. F531L

Description

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl₂. Only template and primers need to be added by the user.

• High fidelity (error rate 4.4 x 10^-7 in Phusion HF Buffer)
• Speed of the polymerase allows short extension times (15-30 s/kb)
• Robust reactions, minimal optimization needed
• Increased product yields with minimal enzyme amounts

Includes:

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl₂ and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl₂ and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Specifications

• Content And Storage: • 10 x 1.25 mL Phusion Master Mix with HF Buffer (2X) (provides 1.5 mM MgCl₂ in final reaction)
  • 2 x 500 μL 100% DMSO
  
  Store at -20°C.
  
  
• GC-Rich PCR Performance: Medium
• Reaction Speed: Fast
• Product Type: High-Fidelity PCR Master Mix
• Sufficient For: 500 x 50 μL Reactions
• Concentration: 2X
• Hot Start: No
• Overhang: Blunt
• Size (Final Product): ≤20 kb

• Format: Tube
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Line: Phusion
• Purity or Quality Grade: DHPLC
• For Use With (Application): Cloning
• Fidelity (vs. Taq): 52 X
• No. of Reactions: 500 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.