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Thermo Scientific™ Phusion High-Fidelity PCR Kit
Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.
$97.00 - $337.00
Specifications
Content And Storage | Includes: • Phusion DNA Polymerase (2 U/µL) • 5X Phusion GC Buffer • 5X Phusion HF Buffer • dNTP mix (10 mM each) • MgCl2 solution (50 mM) • Control template (λ DNA) • 1.3 kb primers (4 µM each) • 10 kb primers (4 µM each) • DNA size standard • DMSO Store at -20°C. |
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Fidelity (vs. Taq) | 52 X |
Format | Kit |
GC-Rich PCR Performance | High |
Hot Start | No |
Catalog Number | Mfr. No. | Includes | Quantity | Price | Quantity & Availability | ||||
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Catalog Number | Mfr. No. | Includes | Quantity | Price | Quantity & Availability | ||||
F553L
|
Thermo Scientific™
F553L |
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS | 200 x 50 μL Reactions (or 500 x 20 μL Reactions) |
Each for $337.00
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F553S
|
Thermo Scientific™
F553S |
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS | 50 x 50 μL Reactions (or 125 x 20 μL Reactions) |
Each for $97.00
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Description
Highlights
- Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
- Polymerase speed allows short extension times (15 to 30 s/kb)
- Robust performance, minimal optimization needed
- Increased product yields with minimal enzyme amounts
- Direct loading on gel with clear Buffer
Specifications
Includes: • Phusion DNA Polymerase (2 U/µL) • 5X Phusion GC Buffer • 5X Phusion HF Buffer • dNTP mix (10 mM each) • MgCl2 solution (50 mM) • Control template (λ DNA) • 1.3 kb primers (4 µM each) • 10 kb primers (4 µM each) • DNA size standard • DMSO Store at -20°C. |
|
Kit | |
No | |
Phusion High-Fidelity DNA Polymerase | |
Fast | |
DNA | |
Phusion | |
PCR Amplification |
52 X | |
High | |
Blunt | |
Separate Components | |
20 kb | |
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS | |
High-Fidelity PCR Kit |
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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