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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

$101.90 - $354.00

Specifications

Content And Storage
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Store at -20°C.
Format Kit
GC-Rich PCR Performance High
Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
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Products 2
Catalog Number Mfr. No. Sufficient For Price Quantity & Availability  
Catalog Number Mfr. No. Sufficient For Price Quantity & Availability  
F553L
View Documents
Thermo Scientific™
F553L
200 x 50 μL Reactions (or 500 x 20 μL Reactions)
Each for $354.00
Add to Cart
 
F553S
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Thermo Scientific™
F553S
50 x 50 μL Reactions (or 125 x 20 μL Reactions)
Each for $101.90
Add to Cart
 
Description

Description

Highlights

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with clear Buffer
Specifications

Specifications


• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Store at -20°C.
High
Fast
High-Fidelity PCR Kit
52 X
Blunt
20 kb
Kit
Phusion High-Fidelity DNA Polymerase
Phusion
Cloning
No
Separate Components
DNA
SDS
Documents

Documents

Product Certifications

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

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