Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Catalog Number	Mfr. No.	Includes	Quantity
F553L	Thermo Scientific™ F553L	Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS	200 x 50 μL Reactions (or 500 x 20 μL Reactions)
F553S	Thermo Scientific™ F553S	Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS	50 x 50 μL Reactions (or 125 x 20 μL Reactions)

Description

Highlights

• Extreme fidelity (error rate 4.4 x 10^-7 in Phusion HF Buffer)
• Polymerase speed allows short extension times (15 to 30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with clear Buffer

Specifications

• Content And Storage: Includes:
  • Phusion DNA Polymerase (2 U/µL)
  • 5X Phusion GC Buffer
  • 5X Phusion HF Buffer
  • dNTP mix (10 mM each)
  • MgCl₂ solution (50 mM)
  • Control template (λ DNA)
  • 1.3 kb primers (4 µM each)
  • 10 kb primers (4 µM each)
  • DNA size standard
  • DMSO
  
  Store at -20°C.
  
• Format: Kit
• Hot Start: No
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Reaction Speed: Fast
• Starting Material: DNA
• Product Line: Phusion
• For Use With (Application): PCR Amplification

• Fidelity (vs. Taq): 52 X
• GC-Rich PCR Performance: High
• Overhang: Blunt
• Reaction Format: Separate Components
• Size (Final Product): 20 kb
• Includes: Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
• Product Type: High-Fidelity PCR Kit

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.