Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Catalog Number	Mfr. No.	Sufficient For
F553L	Thermo Scientific™ F553L	200 x 50 μL Reactions (or 500 x 20 μL Reactions)
F553S	Thermo Scientific™ F553S	50 x 50 μL Reactions (or 125 x 20 μL Reactions)

Description

Highlights

• Extreme fidelity (error rate 4.4 x 10^-7 in Phusion HF Buffer)
• Polymerase speed allows short extension times (15 to 30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with clear Buffer

Specifications

• Content And Storage:
  • Phusion DNA Polymerase (2 U/µL)
  • 5X Phusion GC Buffer
  • 5X Phusion HF Buffer
  • dNTP mix (10 mM each)
  • MgCl₂ solution (50 mM)
  • Control template (λ DNA)
  • 1.3 kb primers (4 µM each)
  • 10 kb primers (4 µM each)
  • DNA size standard
  • DMSO
  
  Store at -20°C.
  
• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Product Type: High-Fidelity PCR Kit
• Fidelity (vs. Taq): 52 X
• Overhang: Blunt
• Size (Final Product): 20 kb

• Format: Kit
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Line: Phusion
• For Use With (Application): Cloning
• Hot Start: No
• Reaction Format: Separate Components
• Starting Material: DNA

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.