Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Catalog Number	Mfr. No.	No. of Reactions
F553S	Thermo Scientific™ F553S	50 Reactions
F553L	Thermo Scientific™ F553L	200 Reactions

Description

Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume.

Highlights

• High fidelity (52X Taq)
• Fast PCR due to short extension times (15–30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amounts

Applications

• High-fidelity PCR
• High throughput
• Amplification of difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning & mutagenesis
• Microarray

Notes

• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Content And Storage: • Phusion DNA Polymerase (2 U/μL)
  • 5X Phusion GC & HF Buffer
  • dNTP mix (10 mM each)
  • Control template (λ DNA) and DNA size standard
  • 1.3 kb & 10 kb primers (4 μM each)
  • 50 mM MgCl₂ solution and DMSO
  
  Store at –20°C.
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Type: High-Fidelity PCR Kit
• For Use With (Application): Standard PCR, High-fidelity PCR
• Hot Start: No
• Reaction Format: Separate Components

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 52X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.