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Thermo Scientific™ Phusion High-Fidelity PCR Kit
Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.
$106.00 - $367.00
Specifications
Content And Storage | • Phusion DNA Polymerase (2 U/µL) • 5X Phusion GC Buffer • 5X Phusion HF Buffer • dNTP mix (10 mM each) • MgCl2 solution (50 mM) • Control template (λ DNA) • 1.3 kb primers (4 µM each) • 10 kb primers (4 µM each) • DNA size standard • DMSO Store at -20°C. |
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Format | Kit |
GC-Rich PCR Performance | High |
Polymerase | Phusion High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Catalog Number | Mfr. No. | Sufficient For | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | Sufficient For | Price | Quantity & Availability | |||||
F553L
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Thermo Scientific™
F553L |
200 x 50 μL Reactions (or 500 x 20 μL Reactions) |
Each for $367.00
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F553S
|
Thermo Scientific™
F553S |
50 x 50 μL Reactions (or 125 x 20 μL Reactions) |
Each for $106.00
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Description
Highlights
- Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
- Polymerase speed allows short extension times (15 to 30 s/kb)
- Robust performance, minimal optimization needed
- Increased product yields with minimal enzyme amounts
- Direct loading on gel with clear Buffer
Specifications
• Phusion DNA Polymerase (2 U/µL) • 5X Phusion GC Buffer • 5X Phusion HF Buffer • dNTP mix (10 mM each) • MgCl2 solution (50 mM) • Control template (λ DNA) • 1.3 kb primers (4 µM each) • 10 kb primers (4 µM each) • DNA size standard • DMSO Store at -20°C. |
|
High | |
Fast | |
High-Fidelity PCR Kit | |
52 X | |
Blunt | |
20 kb |
Kit | |
Phusion High-Fidelity DNA Polymerase | |
Phusion | |
Cloning | |
No | |
Separate Components | |
DNA |
For Research Use Only. Not for use in diagnostic procedures.
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