Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Supplier:  Thermo Scientific™ F553S

Catalog No. F553S

Description

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Highlights
• High fidelity (52X Taq)
• Fast PCR due to short extension times (15-30 s/kb)
• Robust performance, minimal optimization needed
• High yields of PCR products with minimal enzyme amounts
• Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications
• High-fidelity PCR
• Cloning
• Template generation for sequencing
• Amplification of difficult (GC-rich) templates
• Long-range PCR (up to 20 kb)
• Mutagenesis
• High throughput PCR
• Microarray
Thermo Scientific Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume.

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Specifications

• Content And Storage: • Phusion DNA Polymerase (2 U/μL)
  • 5X Phusion GC & HF Buffer
  • dNTP mix (10 mM each)
  • Control template (λ DNA) and DNA size standard
  • 1.3 kb & 10 kb primers (4 μM each)
  • 50 mM MgCl₂ solution and DMSO
  
  Store at –20°C.
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Type: High-Fidelity PCR Kit
• For Use With (Application): Standard PCR, High-fidelity PCR
• Hot Start: No
• Overhang: Blunt
• Size (Final Product): 20 kb or less

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 52X
• No. of Reactions: 50 Reactions
• Reaction Format: Separate Components

For Research Use Only. Not for use in diagnostic procedures.