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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Supplier:  Thermo Scientific™ F553S

 View more versions of this product

Catalog No. F553S


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Description

Description

Highlights

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with clear Buffer
Specifications

Specifications


• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Store at -20°C.
High
Fast
High-Fidelity PCR Kit
Cloning
No
Blunt
20 kb
Kit
Phusion High-Fidelity DNA Polymerase
Phusion
50 x 50 μL Reactions (or 125 x 20 μL Reactions)
52 X
50 Reactions
Separate Components
DNA
SDS
Documents

Documents

Product Certifications
Promotions

Promotions

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Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.