Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Supplier:  Thermo Scientific™ F553S

Catalog No. F553S

Description

Highlights

• Extreme fidelity (error rate 4.4 x 10^-7 in Phusion HF Buffer)
• Polymerase speed allows short extension times (15 to 30 s/kb)
• Robust performance, minimal optimization needed
• Increased product yields with minimal enzyme amounts
• Direct loading on gel with clear Buffer

Specifications

• Content And Storage:
  • Phusion DNA Polymerase (2 U/µL)
  • 5X Phusion GC Buffer
  • 5X Phusion HF Buffer
  • dNTP mix (10 mM each)
  • MgCl₂ solution (50 mM)
  • Control template (λ DNA)
  • 1.3 kb primers (4 µM each)
  • 10 kb primers (4 µM each)
  • DNA size standard
  • DMSO
  
  Store at -20°C.
  
• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Product Type: High-Fidelity PCR Kit
• For Use With (Application): Cloning
• Hot Start: No
• Overhang: Blunt
• Size (Final Product): 20 kb

• Format: Kit
• Polymerase: Phusion High-Fidelity DNA Polymerase
• Product Line: Phusion
• Sufficient For: 50 x 50 μL Reactions (or 125 x 20 μL Reactions)
• Fidelity (vs. Taq): 52 X
• No. of Reactions: 50 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.