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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Manufacturer:  Thermo Scientific™ F553L

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Catalog No. F553L


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Description

Description

Highlights

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with clear Buffer
Specifications

Specifications

Includes:
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Store at -20°C.
Kit
No
Blunt
Separate Components
20 kb
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
High-Fidelity PCR Kit
PCR Amplification
52 X
High
200 Reactions
Phusion High-Fidelity DNA Polymerase
Fast
DNA
Phusion
200 x 50 μL Reactions (or 500 x 20 μL Reactions)
SDS
Documents

Documents

Product Certifications
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Promotions

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