Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Supplier:  Thermo Scientific™ F548L

Catalog No. F548L

Description

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to add template and primers minimizing the number of pipetting steps.

The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features
• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing 'zero-time reactivation'
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time

Applications
• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Specifications

• Content And Storage: Phusion Flash High-Fidelity PCR Master Mix (5 x 1 mL)
  
  Store at -20°C.
• Polymerase: Phusion Flash II DNA Polymerase
• Product Type: High-Fidelity PCR Master Mix
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 25X
• No. of Reactions: 500 Reactions
• Reaction Format: SuperMix or Master Mix

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Concentration: 2X
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.