Invitrogen™ Platinum™ Taq DNA Polymerase, high fidelity

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Includes: Platinum™ Taq DNA Polymerase High Fidelity (20μL, at 5U/μL) is supplied with a vial of 10X High Fidelity Buffer [600mM Tris-SO₄ (pH 8.9), 180mM (NH₄)2SO₄] (1.25mL), and a vial of 50mM MgSO₄ (1mL).

Catalog Number	Mfr. No.	No. of Reactions
11-304-011	Invitrogen™ 11304011	100 Reactions
11-304-029	Invitrogen™ 11304029	500 Reactions
11-304-102	Invitrogen™ 11304102	5000 Reactions

Description

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum™ Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum™ automatic 'hot-start' technology. Features of Platinum™ Taq DNA Polymerase, High Fidelity:

• Fidelity—greater than six times higher fidelity than Taq DNA polymerase
• Amplicon size—amplification of fragments up to 15 kb (see figure)
• Convenience—room temperature reaction assembly
• Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR

Unit definition
One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Order Info

Shipping Condition: Dry ice

Specifications

• Detection Method: Primer-probe
• Polymerase: Platinum Taq DNA Polymerase High Fidelity
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 6X
• Reaction Format: Separate Components

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.