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Invitrogen™ Platinum™ Taq DNA Polymerase, high fidelity
Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.
Supplier: Invitrogen™ 11304029
Includes: Platinum™ Taq DNA Polymerase High Fidelity (20μL, at 5U/μL) is supplied with a vial of 10X High Fidelity Buffer [600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4] (1.25mL), and a vial of 50mM MgSO4 (1mL).
Description
Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum™ Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum™ automatic 'hot-start' technology. Features of Platinum™ Taq DNA Polymerase, High Fidelity:
• Fidelity—greater than six times higher fidelity than Taq DNA polymerase
• Amplicon size—amplification of fragments up to 15 kb (see figure)
• Convenience—room temperature reaction assembly
• Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit definition
One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Order Info
Shipping Condition: Dry ice
Specifications
• Platinum Taq DNA Polymerase High Fidelity (1 x 100 μL at 5 U/μL) • 10X High Fidelity Buffer (1 x 2.25 mL) • 50 mM MgSO4 (1 x 1 mL) Store at -10°C to -30°C. |
|
Low | |
Standard | |
Dry Ice | |
6X | |
500 Reactions | |
Separate Components |
Primer-probe | |
Platinum Taq DNA Polymerase High Fidelity | |
DNA Polymerase High Fidelity | |
Hot-start PCR, High-fidelity PCR | |
Built-In Hot Start | |
Mixed | |
20 kb or less |
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For Research Use Only. Not for use in diagnostic procedures.