Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit

Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.

Catalog Number	Mfr. No.	No. of Reactions
FERK0651	Thermo Scientific™ K0651	10
FERK0652	Thermo Scientific™ K0652	30

Description

Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.

Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.

Highlights

• Non-radioactive labeling of DNA
• Efficient priming of labeling reactions with random decamers
• High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Specifications

• Labeling Target: Genomic DNA, PCR Products, DNA (General)
• No. of Reactions: 10
• Quantity: 10 Reactions