Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit

Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.

Supplier:  Thermo Scientific™ K0651

Catalog No. FERK0651

Description

Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.

Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems.

The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C. Klenow Fragment, exo- is also included in the kit; this genetically engineered enzyme has no detectable exonuclease activity. Therefore, the enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. As a result, DNA fragments of any length can be uniformly labeled. Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or with conventional biotin-avidin or biotin-streptavidin detection systems.

• Non-radioactive labeling of DNA
• Efficient priming of labeling reactions with random decamers
• High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Recommended for:

Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations

Specifications

• Detection Method: Biotin-based
• Final Product Type: DNA
• Includes Label or Dye: Yes
• No. of Reactions: 10
• Product Type: DNA Labeling Kit

• Format: Kit
• Labeling Target: Genomic DNA, PCR Products, DNA (General)
• Labeling Method: Direct Labeling
• Product Line: DecaLabel
• Quantity: 10 Reactions