DescriptionDNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.
- Hybridization stringency can be controlled by elevating temperatures up to 75°C
- Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
- Rapid protocol saves two to three hours over conventional indirect methods
Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2 or 5000cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent
|2°C to 8°C|
|Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes|
|Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent|
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