Cytiva Amersham™ AlkPhos Direct™ Labeling Modules

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.

Supplier:  Cytiva RPN3680

Catalog No. 45-000-936

Description

DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

• Hybridization stringency can be controlled by elevating temperatures up to 75°C
• Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
• Rapid protocol saves two to three hours over conventional indirect methods

Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm² or 5000cm² membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent

Specifications

• Content And Storage: 2°C to 8°C
• For Use With (Application): Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
• Product Type: AlkPhos Direct Labeling Module

• Description: AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.
• Includes: Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent
• Quantity: 2500 cm2 Membrane