Protein Purification Reagents and Kits

Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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1 – 30 of 98 results
1

Cytiva HisTrap™ HP Prepacked Columns
GSA_VA

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Cytiva PD MidiTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL.  Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
3

Cytiva XK Chromatography Columns (Empty)
GSA_VA

Ensure trouble-free operation for low to medium pressure. Cytiva XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.

EDGE
Particle Size 10μm pore size
Product Type XK Chromatography Columns (Empty)
Material Borosilicate glass
For Use With (Application) Liquid chromatography at low to medium pressure
Support Type Polypropylene (reinforced glass fiber)/Polyamide Net
4

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

5

Cytiva Glutathione Sepharose™ 4 Fast Flow Media
GSA_VA

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Particle Size 90 μm
Ligand Type Glutathione and 10-carbon linker arm
Flow Rate <450 cm/hr.
Type Glutathione Sepharose 4 Fast Flow
Content And Storage 4°C to 30°C, 20% Ethanol
Matrix Highly cross-linked 4% agarose
Kit Contents 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
Description Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
Max. Operating Pressure base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
pH Range 3 to 12
Binding Capacity >10 mg/mL
Support Type 4% Highly Cross-linked Agarose
6

Cytiva HisTrap™ FF Columns: 16 × 25mm
GSA_VA

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Promotions are available
Flow Rate <20 mL/min.
Type HisTrap FF
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
7

Cytiva SOURCE™ Tricorn™ Columns for IEX

Source 15Q 4.6/100 PE is a strong anion exchanger prepacked in a Tricorn column for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

Buffer All common ion exchange Buffers
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.18 in.
Bed Volume 1.7mL
Diameter (Metric) Inner 4.6mm
Quantity 1 x 1.7mL
Material PEEK
For Use With (Application) High performance ion exchange chromato graphy of Proteins, Peptides, Polynucleotides and other biomolecules
Max. Pressure 40 bar
Particle Size 15μm
Flow Rate <5mL/min.
Support Type Polystyrene/Divinylbenzene
Height (English) 3.93 in.
8

Cytiva Thrombin Protease
GSA_VA

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

9

Cytiva GSTrap™ 4B Prepacked Columns: 7 × 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 × 25mm are prepacked with Glutathione Sepharose™ 4B

Ligand Type Glutathione
Flow Rate <4 mL/min.
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 4 to 13
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Support Type 4% Highly Cross-linked Agarose
10

Cytiva HiTrap™/HiPrep™ Column Accessories
GSA_VA

Material Polypropylene
11

Cytiva GST Bulk Kit

For small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins

12

Cytiva SOURCE™ 15Q

Strong anion exchanger with high chemical stability for wide range of working conditions with good resistance to cleaning conditions at high pH

Particle Size 15μm
Buffer 20mM N-methylpiperazine, 20mM Piperazine, 20mM Bis-Tris, 20mM Triethanolamine, 20mM Tris, 20mM Diethanolamine, 20mM Ethanolamine, Glycine, 20mM 1,3-diamino-propane, 20mM Piperidine
Ligand Type Quaternary Ammonium
Flow Rate 1800cm/hr.
Height (Metric) 100mm
Content And Storage 4° to 30°C
pH Range 2 to 12
Product Type Source 15Q chromato graphy media
For Use With (Application) Polishing steps in both research and industrial applications
Support Type Polystyrene/Divinylbenzene
Max. Pressure 5 bar
Height (English) 3.93 in.
13

Cytiva Sephacryl™ High Resolution Media

Purify proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale. Cytiva Life Sciences™Sephacryl™ High Resolution Media enables fast and reproducible purifications.

Buffer 0.2M NaOH, 0.1M HCl, 1M Acetic Acid, 8M Urea, 6M Guanidine HCl, 1% SDS, 20% Ethanol, 30% Propanol, 30% Aceto nitrile (tested at 40°C for 1 week), 0.5M NaOH (only for cleaning-in-place)
Height (Metric) 15cm
Content And Storage 4° to 30°C
pH Range 3 to 11
For Use With (Application) Purification of Proteins, Polysaccharides and other macromolecules
Support Type Allyl Dextran and N, N'-methylenebisacrylamide
Max. Pressure 1 bar
Height (English) 5.9 in.
14

Cytiva GSTPrep™ FF 16/10 Column

Fast and easy one-step, scale-up purification of GST-tagged recombinant proteins

15

Cytiva HiScreen Capto Q ImpRes Prepacked Column

HiScreen Capto Q ImpRes columns are prepacked with Capto Q ImpRes ion exchange chromatography medium, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

16

Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Designed for method optimization and parameter screening

Buffer 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <700cm/hr.
Support Type Highly cross-linked Agarose with Dextran surface extender
Height (English) 3.93 in.
17

Cytiva HiTrap Protein L, 5 × 5ml
GSA_VA

Prepacked with Capto L. Cytiva HiTrap Protein L, 5 × 5ml facilitate easy protein purification. They are prefilled with Capto L, a BioProcess chromatography medium that combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand.

18

Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ Fast Flow IEX Columns

Designed for method optimization and parameter screening

Buffer 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Optimization and parameter screening, ion exchange chromato graphy
Max. Pressure 1.5 bar
Particle Size 45 to 165μm
Flow Rate <450cm/hr.
Support Type 6% Cross-linked Agarose
Height (English) 3.93 in.
19

Cytiva HiScreen Prepacked HIC Column, Capto Butyl

Intended for initial and intermediate step purifications that require a medium with low to medium hydrophobicity.

20

Cytiva HiScreen™ Capto™ adhere Multimodal Prepacked Column

For intermediate purification and polishing of monoclonal antibodies after the protein A capture step

21

Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns

Designed for method optimization and parameter screening

Buffer 8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Optimization and parameter screening, ion exchange chromato graphy
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <150cm/hr.
Support Type 6% Cross-linked Agarose
Height (English) 3.93 in.
22

Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Used for method optimization and parameter screening

Particle Size 45 to 165μm
Height (Metric) 100mm
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
Support Type 6% Cross-linked Agarose
Max. Pressure 1.5 bar
Height (English) 3.93 in.
23

Cytiva HiScreen Phenyl FF (High Sub)

HiScreen Phenyl FF (high sub) columns are prepacked with Phenyl Sepharose 6 Fast Flow hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.

24

Cytiva HiScreen™ Sepharose™ Prepacked High Performance HIC Columns

Designed for method optimization and parameter screening

Particle Size 24 to 44μm
Flow Rate <150cm/hr.
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
pH Range 3 to 13
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Convenient process development optimization parameter screening
Max. Pressure 3 bar
Height (English) 3.93 in.
25

Cytiva Capto™ Core 700
GSA_VA

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.

Particle Size 90μm
Buffer 1M NaOH, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Ligand Type Octylamine
Flow Rate <500cm/hr.
Content And Storage 4° to 30°C
pH Range 2 to 14
Product Type Capto Core 700 chromato graphy media
For Use With (Application) Intermediate purification and polishing of viruses and other large biomolecules
Support Type Highly Cross-linked Agarose
26

Cytiva HiScreen Capto Blue

Excellent for method optimization and parameter screening due to the 10 cm bed height

27

Cytiva Capto™ L Chromatography Medium
GSA_VA

Capto L is a BioProcess chromatography medium (resin) for the capture of antibodies and antibody fragments.

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Flow Rate <500cm/hr.
Height (Metric) 200mm
Content And Storage 2° to 8°C
pH Range 2 to 10
Product Type Capto L chromato graphy media
For Use With (Application) High binding capacity, low ligand leakage and selectivity for a broad range of Antibody fragments reduces process time and amount of medium
Support Type Highly Cross-linked Agarose
Height (English) 7.87 in.
28

Cytiva HiTrap™ Capto™ Core 700 Columns

HiTrap Capto Core 700 is prepacked with Capto Core 700, a multimodal BioProcess medium for intermediate purification and polishing of viruses and other large biomolecules.

29

Cytiva HiTrap™ Protein L Prepacked Columns

For capture of antibodies and antibody fragments such as Fab fragments, scFv, and Dabs

Particle Size 85μm
Ligand Type Recombinant Protein L (E. coli), mammalian free
Flow Rate <500cm/hr.
Height (Metric) 20mm
Content And Storage 2° to 8°C
pH Range 2 to 10
Product Type HiTrap Protein L column
Material Polypropylene
For Use With (Application) Affinity chromato graphy medium (Resin) for capture of Antibodies and Antibody fragments such as Fab fragments, scFv and Dabs
Support Type Highly Cross-linked Agarose
Max. Pressure 5 bar
Height (English) 0.78 in.
30

Cytiva Superose™ 6 Increase Columns
GSA_VA

High-resolution size-exclusion chromatography for studying large proteins and complexes.   Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Material Borosilicate glass
Max. Pressure 435 psi