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Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.
Height (Metric) | 25 mm |
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For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Use for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MiniTrap™ G-10 Sample Preparation Columns are prepacked, single-use gravity columns.
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
Type | Pre-packed Column |
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Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Particle Size | 90μm |
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Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Ensure trouble-free operation for low to medium pressure. Cytiva XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.
Particle Size | 10μm pore size |
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Product Type | XK Chromatography Columns (Empty) |
Material | Borosilicate glass |
For Use With (Application) | Liquid chromatography at low to medium pressure |
Support Type | Polypropylene (reinforced glass fiber)/Polyamide Net |
Affinity chromatography resin designed for fast and reliable, high resolution purification of biotinylated biomolecules.
The HiTrap™ Capto™ MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).