Protein Purification Reagents and Kits
Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva PD MiniTrap™ G-10 Sample Preparation Columns
Use for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. Cytiva Life Sciences™ PD MiniTrap™ G-10 Sample Preparation Columns are prepacked, single-use gravity columns.
Cytiva PD MidiTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Particle Size | 90μm |
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Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
Flow Rate | 150 to 600cm/hr. |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | Ni Sepharose excel chromato graphy media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva XK Chromatography Columns (Empty)
Ensure trouble-free operation for low to medium pressure. Cytiva XK Chromatography Columns (Empty) connect directly to ÄKTA design instruments or other liquid chromatography systems. The user-friendly ensures reproducible, accurate packing.
Particle Size | 10μm pore size |
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Product Type | XK Chromatography Columns (Empty) |
Column Type | Empty Chromatography Column |
Material | Borosilicate glass |
For Use With (Application) | Liquid chromatography at low to medium pressure |
Support Type | Polypropylene (reinforced glass fiber)/Polyamide Net |
Cytiva Glutathione Sepharose™ 4B Media
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Particle Size | 90 μm |
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pH Range | 4 to 13 |
Material | Glutathione Sepharose 4 B |
Cytiva LabMate™ PD-10 Buffer Reservoirs
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
Particle Size | 90μm |
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Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
Flow Rate | 600cm/hr. |
Height (Metric) | 250mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | Ni Sepharose 6 FF chromatography media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Cross-linked Agarose |
Max. Pressure | 1 bar |
Height (English) | 9.84 in. |
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
Type | Pre-packed Column |
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Content And Storage | 4°C to 30°C |
Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 2 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva™ HiTrap™ Capto™ Adhere
The HiTrap Capto adhere is prepacked with a strong anion exchange multimodal resin for removing contaminants in post-protein A purification.
Cytiva™ HiTrap™ Capto™ Adhere ImpRes
The HiTrap™ Capto™ Adhere ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere ImpRes strong anion exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).
Cytiva GST Gene Fusion System pGEX Vectors
Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schistosoma japonicum GST
Type | pGEX Vector |
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Quantity | 25 μg |
For Use With (Application) | Designed for inducible, high-level intracellular expression of genes or gene fragments as fusions with Schisto soma japonicum GST |
Cytiva HiScreen Prepacked HIC Column, Capto Butyl
Intended for initial and intermediate step purifications that require a medium with low to medium hydrophobicity.
Cytiva HiTrap Capto MMC Columns
HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange medium. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | HiTrap Capto MMC column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Bind Proteins at the conductivity of the feed material and to solve specific purification challenges |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva HiTrap™ Chelating HP Columns
Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.
Particle Size | 34μm |
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Buffer | 6M Guanidine Hydrochloride, 8M Urea |
Ligand Type | Iminodiacetic Acid |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 13 |
Product Type | HiTrap chelating HP column |
Column Type | Affinity Column |
For Use With (Application) | IMAC and subsequent purification of Polyhistidine tagged Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 3 bar |
Height (English) | 0.98 in. |