Gel Shift Assays
Gel Shift Assays
Enzo Life Sciences PROTEOSTAT Thermal shift stability assay kit (1x400tests)
Accelerated screening for protein stability as a function of pH, ionic strength, and concentration. The PROTEOSTAT Thermal shift stability assay kit includes a fluorescent dye which detects protein aggregation, so it can be used to monitor protein stability under systematic thermal stress conditions. From the thermal shift assay, a temperature at which the bulk of the protein becomes aggregated can readily be identified. The aggregation temperature is an indicator of protein stability and can be used to optimize conditions that minimize protein aggregation as well as to identify ligands that bind and confer structural stability to a protein of interest. Use the related PROTEOSTAT Protein Refolding and Aggregation Sensing Kit (Prod. No. ENZ-51040) to identify optimal protein refolding conditions. Conditions that increase the aggregation temperature, increase the stability of the protein. Contents: PROTEOSTAT TS Detection Reagent. β-Lactoglobulin control. 10X Assay Buffer
