Test
A red-excitable, pH-sensitive cyanine dye derivative, reporting receptors movement from the cell surface into acidic endosomes upon agonist stimulation.
Product Type | CypHer5E NHS Ester |
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Formulation | 100% CypHer 5E |
Color | Blue |
Solubility | Easily soluble in methanol; Very slightly soluble in cold water and hot water. |
Emission | 663 |
For Use With (Application) | This reagent has been shown to be suitable for live cell receptor internalization studies using the IN Cell Analyzer 3000, IN Cell Analyzer 1000, and confocal microscopes. 0.5 mL of stock reagent will be sufficient for up to 500 wells. |
Highly suitable for both analytical and preparative gels. DeStreak Rehydration Solution improves reproducibility and quality of 2-D gels by preventing streaking.
Content And Storage | 2°C to 8°C |
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Kit Contents | Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent |
pH Range | >7 |
Product Type | Destreak Reagent |
For Use With (Application) | Improves reproducibility and quality of 2D Gels by preventing streaking |
Convenient way to tag antibodies with CyDye fluorescent dyes
Content And Storage | 2°C to 8°C, protected from light |
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pH Range | 7 to 7.3 |
Quantity | Each |
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. Amersham CyDye™ DIGE Fluor Minimal Labeling Kits contain size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan DIGE system.
Content And Storage | -15°C to -30°C |
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Format | Solid |
Color | Blue, Green, Red |
Designed for use in 2-D Fluorescence Difference Gel Electrophoresis using the Ettan DIGE system. Cytiva Amersham CyDye DIGE Fluor minimal dyes and saturation dyes are size- and charge-matched fluorescent dyes specifically engineered for detecting protein abundance differences.
Combines exceptional photostability with bright and intense signals. Cytiva Cy™3B NHS Ester Mono-Reactive CyDye™ provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications.
Format | Solid |
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Product Type | Cy3B NHS Ester |
Molecular Weight (g/mol) | 771 |
Formulation | 100% Cy3B (NHS ester) |
Color | Red |
Solubility | Soluble in aqueous buffers |
Emission | 570 |
Purity | >99% |
Excitation | 559 |
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
Content And Storage | -15°C to -30°C |
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Format | Solid |
Product Type | Amersham CyDye DIGE Fluor Cy3 Minimal Dye |
Color | Green |
For Use With (Application) | For general 2-D applications where sufficient amounts of sample are available, detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel |
Amersham QuickStain enables protein detection directly after SDS-PAGE or transfer. Supports Western blot normalization for quantitative Western blotting.
Use for highly accurate labeling of amine groups. Cytiva Amersham™ CyDye™ Value Packs (mono-Reactive NHS Ester) contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
Kit Contents | Foil packs, each containing 1, 5, 10, 25 or 50mg dried dye |
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pH Range | 3 to 10 |
Use for highly accurate labeling of amine groups. Cytiva Cy5.5 NHS Ester Mono-Reactive CyDye contain derivitized CyDyes that carry only one reactive group on each dye molecule for accurate labeling of amine groups, for example on antibodies, peptides, or oligonucleotides.
Kit Contents | Foil packs of premeasured dye |
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Format | Powdered |
pH Range | 3 to 10 |
Product Type | Cy5.5 NHS Ester |
Color | Blue |
Label or Dye | Cy5.5 |
More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Amersham™ Cy™3, Cy™ 5 Maleimide mono-Reactive Dye packs are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.
Content And Storage | 2°C to 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles. |
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Kit Contents | Five vials of dye |
Solubility | Soluble in cold water and hot water |
Sufficient For | Labeling 1mg Protein |
Quantity | 5 vials each sufficient to label 1 mg protein |
Label proteins, antibodies and more. Cytiva Amersham CyDye Value Packs (bis-Reactive NHS Ester) provide maximum economy in general labeling applications. Available in a variety of reactive dye colors: red (Cy3), blue (Cy5.5) and purple (Cy7).
For the selective labeling of free sulfhydryl groups such as cysteine residues in peptides, proteins, and oligonucleotides
Kit Contents | Foil packs, each containing 1, 5 or 25mg dried dye |
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pH Range | 3 to 10 |
Label proteins, antibodies and more. Cytiva Amersham CyDye Value Packs (bis-Reactive NHS Ester) provide maximum economy in general labeling applications. Available in a variety of reactive dye colors: red (Cy3), blue (Cy5.5) and purple (Cy7).