Cytiva DeStreak Rehydration Solution

Highly suitable for both analytical and preparative gels. DeStreak Rehydration Solution improves reproducibility and quality of 2-D gels by preventing streaking.

Catalog Number	Mfr. No.	Quantity
45-000-380	Cytiva 17600318	1 mL
45-000-381	Cytiva 17600319	5 x 3 mL

Description

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs. DeStreak Rehydration Solution effectively eliminates nonspecific oxidation by maintaining protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.

• Eliminates extra spots caused by nonspecific oxidation of proteins
• Stabilizes proteins throughout entire 2-D runs, ensuring simplified, well-resolved protein patterns
• Suitable for both analytical and preparative gels
• Contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak Reagent

Order Info

The solution is ready for use after addition of the appropriate IPG Buffer. DeStreak Reagent is also available separately in a 1mL pack size.

Specifications

• For Use With (Application): Improves reproducibility and quality of 2D Gels by preventing streaking
• Kit Contents: Optimized concentrations of urea, thiourea, CHAPS and Destreak reagent
• Content And Storage: 2°C to 8°C

• Product Type: Destreak Reagent
• pH Range: >7