Test
NC1789154 NITTOPHASE HL UNYLINKE 350 50G
PMM_1609-04; KIT, NO MODIFIER/ COND SRV, "A" -20, MFG THERMO FISHER SCIENTIFIC, MFG P/N 314219G10
NC1903226 3-PT-AMINO-MODIFIER C3 CPG 1G
NC1789150 NITTOPHASE HL UNYLINKE 350 10G
3'-Amino-Modifier Serinol CPG, 1x10um
NC2741362 DMT-DC AC LCAA CPG 1000A 20G
NC2875564 NPHL PHT-C6 250 25G
NPHL-UL350, 300g
NC2120234 CLEAVAGE CHUCK ASSEMBLY M192E
PDC 70 Piezo Dispense CapillaryS5,11,SX,DW,S3 (4x manifold)Fixed drop volume between 300-360 pl
Proteinase K is a nonspecific serine protease. It is not inactivated by metal ions, chelating agents (e.g. EDTA), sulfyhydryl reagents or by trypsin or chymotrypsin inhibitors. It is stable over a wide pH (4-12.5), with optimal activity at pH 6.5-9.5. Proteinase K activity can be stimulated by addition of denaturing agents SDS and urea. The optimum temperature for the enzyme is 65C; it is twelve times more active at 65C than at 25C. However, rapid denaturation of the enzyme will occur at temperatures above 65C. Thus, protein digestion is usually carried out at 55C. Autolysis of the enzyme occurs increasingly at alkaline pH. However, proteinase K is not completely inactivated by autolysis, and some enzyme fragments maintain proteolytic activity even after extensive autolysis
Proteinase K is a nonspecific serine protease. It is not inactivated by metal ions, chelating agents (e.g. EDTA), sulfyhydryl reagents or by trypsin or chymotrypsin inhibitors. It is stable over a wide pH (4-12.5), with optimal activity at pH 6.5-9.5. Proteinase K activity can be stimulated by addition of denaturing agents SDS and urea. The optimum temperature for the enzyme is 65°C; it is twelve times more active at 65°C than at 25°C. However, rapid denaturation of the enzyme will occur at temperatures above 65°C. Thus, protein digestion is usually carried out at 55°C. Autolysis of the enzyme occurs increasingly at alkaline pH. However, proteinase K is not completely inactivated by autolysis, and some
NC2741363 DMT-DA BZ LCAA CPG 1000A 20G
3'-Amino-Modifier C7 CPG 1000, 1x10um
3'-Amino Modifier TFA Amino C-6 lcaa CPG 500A 1g