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Highly sensitive, homogeneous fluorescent apoptosis assay for use in cultured cells and with purified caspase. Just add-mix-read.
Format | Cuvets, 96- or 384-well Plates |
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Detection Method | Fluorescence |
A simple, non-lytic assay that allows real-time monitoring of apoptosis progression, without the need for multiple plates or complicated processing. A multimode plate reader for detecting luminescence and fluorescence is the only instrument required.
Staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes.
Contains apoptotic program characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA
Staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic processes
Cells with active caspases can be detected. BD Pharmingen™ Violet Live Cell Caspase Probe contains a fluorochrome-labeled caspase inhibitor. Fluorochrome-labeled caspase inhibitors have three functional domains: fluorochrome , 3-amino acid sequence (VAD) that binds to the active site of the activated caspase, and fluoromethyl ketone (FMK) moiety that allows the probe to irreversibly bind the active caspase.
Designed to measure caspase-3 or DEVD-cleaving activity an marker of cells undergoing apoptosis.
Caspase-8 Inhibitor Screening Kit is used for screening caspase inhibitors using fluorometric methods.
Component for measuring active Caspase-3 and -7 in a homogeneous format.
Easily distinguish cells undergoing apoptosis with Vybrant Apoptosis Assay Kits, which offer dyes, including YO-PRO-1 and Hoechst 33342, for apoptotic and necrotic cell staining.
Format | Tube |
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For Use With (Equipment) | Flow Cytometer |
Storage Requirements | Store in refrigerator (2–8°C) and protect from light |
Detection Method | Fluorescence |
Screen apoptotic cells with Click-iT TUNEL Assay kits, which offer easy Alexa Fluor dye incorporation and can be multiplexed with GFP and RFP.
Shipping Condition | Dry Ice |
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No. of Reactions | 1 X 96 tests or 50 coverslips |
Format | 96-well Plate |
For Use With (Equipment) | Fluorescence Microscope, High Content Instrument |
Product Type | Imaging Assay |
Label Type | Alexa Fluor™ Dyes, Classic Dyes |
Detection Method | Fluorescence |
Product Line | Click-iT |
Fluorogenic, no-wash indicator for live- and fixed-cell applications
Cells with active caspases can be detected. BD Pharmingen™ Yellow-Green Live Cell Caspase Probe contains a fluorochrome-labeled caspase inhibitor. Fluorochrome-labeled caspase inhibitors have three functional domains: fluorochrome , 3-amino acid sequence (VAD) that binds to the active site of the activated caspase, and fluoromethyl ketone (FMK) moiety that allows the probe to irreversibly bind the active caspase.
For detection of Cathepsin L from human and mouse samples