Test
Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 107 transformants per μg of plasmid DNA.
Provides all of the reagents required to perform a quick and easy transformation. Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes it great for the high-efficiency transformation needed in two hours.
Designed for high-throughput transformation with efficiencies up to 103 transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation.
The Multi-Copy Pichia Expression Kit contains the pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of the gene of interest.
The EasySelect™ Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris.
The PichiaPink™ Secretion Optimization Kit is a new recombinant protein expression system based on the yeast Pichia pastoris.
The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene.
High-level E. coli expression using three-minute transformation protocol
Quantity | 20 x 50 μL |
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For Use With (Application) | High-level E. coli expression using 3 min. transformation protocol |
The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108–109 transformants/µg of pUC19 DNA but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Mix & Go preparation method and demonstrate fast transformation kinetics.The procedures are easy. Simply culture the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
Version II further improves the efficiency and reliability ofits predecessor. The competent yeast cells prepared with these reagents can be used immediately for transformation experiments or can be stored frozen at or below -70C for direct use in the future. This method is suitable for both circular and linear plasmid transformations.
631851 \ Quick & Easy Yeast Transformation Mix \ 20 Rxns
Frozen-EZ Solution 1 (60 ml) for use with the Frozen-EZ Yeast Transformation II Kit for yeast transformations.
NC2739969 TRUE BLUE TRANSFORM LAB GEB
Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights Simple and controlled autolysis of E. coli Strains can by lysed in minutes after harvesting Method compatible with most buffer systems Ideal for protein expression and purification, also applicable for extraction of nucleic acids Scale up to lyse more samples without increase in time or labor