Test
Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 10&superscript_7; transformants per µg of plasmid DNA.
Provides all of the reagents required to perform a quick and easy transformation. Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes it great for the high-efficiency transformation needed in two hours.
Designed for high-throughput transformation with efficiencies up to 10&superscript_3; transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation.
High-level E. coli expression using three-minute transformation protocol
Quantity | 20 x 50 μL |
---|---|
For Use With (Application) | High-level E. coli expression using 3 min. transformation protocol |
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates. The master mix format simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components, and yields robust, rapid ligation of all types of DNA ends using a short incubation time at room temperature. No thawing is necessary as it remains liquid during storage at -20C. Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution.
Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights Simple and controlled autolysis of E. coli Strains can by lysed in minutes after harvesting Method compatible with most buffer systems Ideal for protein expression and purification, also applicable for extraction of nucleic acids Scale up to lyse more samples without increase in time or labor
The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Mix & Go cells and the mixture spread directly to a culture plate. Transformation efficiencies typically range from 108–109 transformants/µg of pUC19 DNA but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Mix & Go preparation method and demonstrate fast transformation kinetics.The procedures are easy. Simply culture the E. coli strain of your choice in ZymoBroth™ medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
Version II further improves the efficiency and reliability ofits predecessor. The competent yeast cells prepared with these reagents can be used immediately for transformation experiments or can be stored frozen at or below -70C for direct use in the future. This method is suitable for both circular and linear plasmid transformations.
The CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) quantifies colonies with no manual counting required. Cells are incubated in soft agar for 7-8 days and then quantified using the included fluorescent dye.
ZymoBroth (ZB) is a specially formulated growth medium used for the preparation of highly competent E. coli cells for DNA transformation.
Cells; XL1-Blue MR Supercompetent; Stratagene; For cloning w/o F episome, greater-than-equal-to 1 x 109 transformants/microgram; Deficient in all known E. coli K12 restriction systems; Increase efficiency of introducing eukaryotic DNA
Additionally, it does not require use of any potentially interferingcomponents such as detergents, commonly found in various lytic buffers.Highlights• Simple and controlled autolysis of E. coli• Strains can by lysed in minutes after harvesting• Method compatible with most buffer systems• Ideal for protein expression and purification, also applicable for extraction of nucleic acids• Scale up to lyse more samples without increase in time or labor
Z-Competent Transformation Kit with SOBThe Z-Competent E. coli Transformation Kit is a simple, highly efficient method for preparing cells for transformation. Simply grow cells in SOB medium, wash and resuspend the cells in the Competent Buffer, then the cells are ready to receive DNA. Specifications:300 preps plus 100 ml SOB medium. No heat shock or any other manipulation is required. Routinely produces 2 x 108 to 1 x 109 transformants per 1µg supercoiled pUC19 DNA.
PEG-LIOAC SOLUTION 250 ML, BP-257. 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 100 mM Lithium acetate, and 40% PEG 3350. Prepared in 18.2 megohms water and filtered through 1 micron filter.. This item has a minimum order quantity of 5 per supplier requirements for all the buffers.
Frozen-EZ Solution 3 (60 ml) for use with the Frozen-EZ Yeast Transformation II Kit for yeast transformations.