Test
Over the past few years, SNAP-Tag technology combined with TR-FRET has paved the way for the development of many non-radioactive, no-wash, binding assays. The method is based on transfecting cells using plasmids encoding a SNAP-Tag and subsequently labeling them with Terbium. Cisbio offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready for protein expression and labeling. All information on this page pertains to the Tag-lite plasmid cloned with the V2 Arginine vasopressin 2 receptor.
Z-FR-R110 (Rhodamine 110, bis-(N-CBZ-L-phenylalanyl-L-arginine amide), dihydrochloride) is a substrate for the cysteine proteases cathepsins B and L. The substrate has been used in flow cytometry studies of human monocytes and rat macrophages.