Test
Introduces the principles of extracting plasmid DNA from bacterial cells.
To clone a DNA fragment in pUC-linker and select colonies that have DNA inserts based on color selection.
To study gene structure, function, and to enhance gene expression.
In this experiment, students will read DNA sequences obtained from automated DNA sequencing techniques. The data will be analyzed using publicly available databases to identify the bacterial species present in a patient sample. The results will be used to make a diagnosis.
Students will get to learn the effect of mutations in p53 tumor suppressor gene and its role in familial cancers.