Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Edvotek™ Construction and Cloning of DNA Recombinant
SCI_ED

To study gene structure, function, and to enhance gene expression.

Supplier:  Edvotek™ 301

Catalog No. S11301ND


Add to Cart

Includes: Ultra-pure Water, DNA Fragments for Ligation, T4 DNA Ligase in Reaction Tube, Kanamycin Sulfate, Supercoiled Control DNA Samples, JM109 BactoBeads™, 0.05 M Calcium Chloride, ReadyPour™ LB Agar, Recovery Broth, Growth Medium, Tris-EDTA-Glucose Buffer (TEG), 2M NaOH, 10% SDS, RNase (DNase-free), Tris-EDTA Buffer, Acidified Potassium Acetate, Ultra-pure Water for Restriction Digests, 10X Restriction Enzyme Reaction Buffer, Dryzymes™ Rehydration Buffer, DNA Standard Marker, Supercoiled Plasmid Vector Standard, EcoRI Dryzymes™, PvuII Dryzymes™, XhoI Dryzymes™, InstaStain™ Ethidium Bromide, 10X Gel Loading Solution, 50X Electrophoresis Buffer, UltraSpec-Agarose, Screw Cap Tubes (sterile), Microtest tubes (0.5 ml), petri plates (sterile, 60 x 15mm), 1.5 ml microcentrifuge tubes, sterile inoculating loops/needles, 10ml pipets (sterile), sterile 50 ml culture tubes, petri plates (sterile, 100 x 15 mm)

Description

Description

 

  • It is divided into five modules
  • Clones are constructed by ligation of a vector and a fragment insert
  • The constructs are then transformed into competent cells and the cells are grown and selected for resistance
  • Plasmid DNA is then isolated from the transformants, cleaved with restriction enzymes, and analyzed by agarose gel electrophoresis
  • Recommended for college level courses
  • For 5 lab groups
  • Time: Enzyme digestion 30 min., Gel preparation 30 min., Electrophoresis 45 min., Staining 15 min. and Destaining 20 min.

 

Unit comprises: Electrophoresis tank and power supply, automatic micropipet with tips, balance, microwave or hot plate, waterbath, large weigh boats for staining, UV transilluminator, floating racks for microtest tubes, pipet pump or bulb, 5 or 10mL pipets, laboratory glassware, metric rulers, distilled water and ice

Specifications

Specifications

Electrophoresis apparatus
To study gene structure, function and to enhance gene expression
Instructions, Enzymes, plasmid DNA, restriction Enzyme dilution Buffer, Enzyme grade Water, standard DNA fragments, restriction Enzyme Reaction Buffer, Gel loading solution, Agarose powder, electrophoresis Buffer, stains, calibrated pipet
Product Suggestions

Product Suggestions

SDS
Documents

Documents

Product Certifications
Promotions

Promotions

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit