All Primary Antibodies
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All Primary Antibodies
Primary antibodies, immunoglobulins that bind to specific proteins or other biomolecules, are used in many research applications and protocols to detect targets of interest. They are developed using different animal hosts, including mouse, rat, rabbit, goat, sheep, and many others.
Monoclonal and Polyclonal Antibodies
One type of primary antibodies, monoclonal antibodies, provides high reproducibility and low cross-reactivity and background noise. Another type, polyclonal antibodies, often costs less and provides greater affinity and quicker binding. Both are produced using plasma B cells, but the former uses the same clone and the latter uses different clones. Monoclonal antibodies require hybridoma cell lines, and polyclonal antibodies do not.
There are also recombinant monoclonal antibodies with similar benefits, like high affinity, scalability, and specificity. They are produced using in vitro cloning of plasma B cells and expression hosts.
Conjugated Primary Antibodies
Antibodies can be labeled with various fluorophores or detection agents or used without labels. Labeled primary antibodies, also known as conjugated primary antibodies, help researchers simplify and streamline their applications. They are coupled with common enzymes and dyes such as Alexa Fluor and often used in protein and cell analysis.
Applications
Antibodies for life sciences applications are used in flow cytometry, western blotting, ELISA, immunohistochemistry, and immunocytochemistry. Secondary antibodies can be added to support the detection and purification of certain antigens. They bind to the primary antibody, which binds to the antigen of interest. Finding the right combination of antibodies can result in greater antigen specificity and a strong, detectable signal.
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Filtered Search Results
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Measles nucleoprotein Antibody (83KKII) - BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody has been used in 2 publications
PTK7/CCK4 Antibody (OTI2E7), Novus Biologicals™
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Mouse Monoclonal Antibody
AIF-1/Iba1 Antibody, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 192 publications
Content And Storage | Store at -20C. Avoid freeze-thaw cycles. |
---|---|
Target Species | Human,Mouse,Rat,Canine,Feline |
Host Species | Goat |
Conjugate | Unconjugated |
Applications | Western Blot,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Paraffin),Immunohistochemistry (Frozen),ELISA,Immunohistochemistry (Free Floating),Immunoassay |
Isotype | IgG |
Gene Accession No. | P55008 |
Research Discipline | Autoimmune Diseases, Cancer, Cell Biology, Cell Cycle and Replication, Immunology, Inflammation, Lipid and Metabolism, Microglia Markers, Neuroscience |
Concentration | 0.5 mg/mL |
Antigen | AIF-1/Iba1 |
Gene Symbols | AIF1 |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Gene Alias | AIF-1, allograft inflammatory factor 1, G1, IBA1, IbaI, interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, IRT1, IRT-1, Protein G1 |
Gene ID (Entrez) | 199 |
Formulation | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA with 0.02% Sodium Azide |
Immunogen | This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3. |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. |
S-Tag Epitope Tag Antibody - BSA Free, Novus Biologicals™
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Rabbit Polyclonal Antibody
Antigen | S-Tag Epitope Tag |
---|---|
Regulatory Status | RUO |
Content And Storage | Store at 4C. Do not freeze. |
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Western Blot,ELISA,Immunocytochemistry,Immunofluorescence,Immunoprecipitation |
Classification | Polyclonal |
Immunogen | Rabbits were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
Primary or Secondary | Primary |
SCF/c-kit Ligand Antibody, DyLight 594, Novus Biologicals™
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Rabbit Polyclonal Antibody
CD24 Antibody (ML5) - Azide and BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody
Content And Storage | Store at 4°C. Do not freeze. |
---|---|
Conjugate | Unconjugated |
Target Species | Human |
Host Species | Mouse |
Applications | Flow Cytometry,Immunohistochemistry,Immunofluorescence,Immunohistochemistry (Frozen) |
Form | Purified |
Isotype | IgG2a κ |
Antigen | CD24 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified |
Gene Alias | CD 24, CD24 antigen, CD24 antigen (small cell lung carcinoma cluster 4 antigen), CD24 molecule, CD24Asignal transducer CD24, FLJ22950, FLJ43543, MGC75043, Small cell lung carcinoma cluster 4 antigen |
Formulation | PBS |
Gene ID (Entrez) | 100133941 |
Classification | Monoclonal |
Immunogen | The immunogen was the full length version of the protein: CD24 (ML5) |
Primary or Secondary | Primary |
Clone | ML5 |
GFP Antibody, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 86 publications
Content And Storage | Store at -20°C. Avoid freeze-thaw cycles. |
---|---|
Target Species | Non-species specific |
Host Species | Goat |
Conjugate | Unconjugated |
Applications | Western Blot,Flow Cytometry,ELISA,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunoprecipitation |
Isotype | IgG |
Gene Accession No. | P42212 |
Concentration | 1.1 mg/ml |
Antigen | GFP |
Gene Symbols | GFP |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Dilution | Western Blot 1:1000-1:10000, Flow Cytometry 1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Proximity Ligation Assay, Electron Microscopy 1:10 - 1:500, Immunohistochemistry Free-Floating 1:10 - 1:500, Knockout Validated, Knockdown Validated |
Gene Alias | Enhanced Green Fluorescent Protein, Green Fluorescent Protein, green fluorescent protein (gfp) |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide |
Immunogen | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. (Uniprot: P42212) |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | No reaction was observed against Human, Mouse or Rat serum proteins. |
Collagen Type II Mouse anti-Chicken, Human, Mouse, Salamander, Clone: 6B3, MilliporeSigma™
Mouse Monoclonal Antibody
CD3 epsilon Antibody (BL-298-5D12), Novus Biologicals™
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Rabbit Monoclonal Antibody
beta-III Tubulin Antibody, Novus Biologicals™
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Chicken Polyclonal Antibody has been used in 16 publications
CD31/PECAM-1 Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody has been used in 57 publications
Calcitonin R Antibody (31/01-1H10-4-1-14) - BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody