All Primary Antibodies
All Primary Antibodies
Primary antibodies, immunoglobulins that bind to specific proteins or other biomolecules, are used in many research applications and protocols to detect targets of interest. They are developed using different animal hosts, including mouse, rat, rabbit, goat, sheep, and many others.
Monoclonal and Polyclonal Antibodies
One type of primary antibodies, monoclonal antibodies, provides high reproducibility and low cross-reactivity and background noise. Another type, polyclonal antibodies, often costs less and provides greater affinity and quicker binding. Both are produced using plasma B cells, but the former uses the same clone and the latter uses different clones. Monoclonal antibodies require hybridoma cell lines, and polyclonal antibodies do not.
There are also recombinant monoclonal antibodies with similar benefits, like high affinity, scalability, and specificity. They are produced using in vitro cloning of plasma B cells and expression hosts.
Conjugated Primary Antibodies
Antibodies can be labeled with various fluorophores or detection agents or used without labels. Labeled primary antibodies, also known as conjugated primary antibodies, help researchers simplify and streamline their applications. They are coupled with common enzymes and dyes such as Alexa Fluor and often used in protein and cell analysis.
Applications
Antibodies for life sciences applications are used in flow cytometry, western blotting, ELISA, immunohistochemistry, and immunocytochemistry. Secondary antibodies can be added to support the detection and purification of certain antigens. They bind to the primary antibody, which binds to the antigen of interest. Finding the right combination of antibodies can result in greater antigen specificity and a strong, detectable signal.
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Filtered Search Results
ABCA4 Antibody (3F4), Novus Biologicals™
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Mouse Monoclonal Antibody has been used in 4 publications
AIF-1/Iba1 Antibody, Novus Biologicals™
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Goat Polyclonal Antibody has been used in 192 publications
Content And Storage | Store at -20C. Avoid freeze-thaw cycles. |
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Target Species | Human,Mouse,Rat,Canine,Feline |
Host Species | Goat |
Conjugate | Unconjugated |
Applications | Western Blot,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Paraffin),Immunohistochemistry (Frozen),ELISA,Immunohistochemistry (Free Floating),Immunoassay |
Isotype | IgG |
Gene Accession No. | P55008 |
Research Discipline | Autoimmune Diseases, Cancer, Cell Biology, Cell Cycle and Replication, Immunology, Inflammation, Lipid and Metabolism, Microglia Markers, Neuroscience |
Concentration | 0.5 mg/mL |
Antigen | AIF-1/Iba1 |
Gene Symbols | AIF1 |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Gene Alias | AIF-1, allograft inflammatory factor 1, G1, IBA1, IbaI, interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, IRT1, IRT-1, Protein G1 |
Gene ID (Entrez) | 199 |
Formulation | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA with 0.02% Sodium Azide |
Immunogen | This AIF-1/Iba1 Antibody was developed against a peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3. |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | This AIF-1/Iba1 Antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3). AIF-1/Iba1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. |
CLEC9a Antibody (8F9) - Azide and BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody
CD24 Antibody (ML5) - Azide and BSA Free, Novus Biologicals™
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Mouse Monoclonal Antibody
Content And Storage | Store at 4°C. Do not freeze. |
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Conjugate | Unconjugated |
Target Species | Human |
Host Species | Mouse |
Applications | Flow Cytometry,Immunohistochemistry,Immunofluorescence,Immunohistochemistry (Frozen) |
Form | Purified |
Isotype | IgG2a κ |
Antigen | CD24 |
Regulatory Status | RUO |
Purification Method | Protein A or G purified |
Gene Alias | CD 24, CD24 antigen, CD24 antigen (small cell lung carcinoma cluster 4 antigen), CD24 molecule, CD24Asignal transducer CD24, FLJ22950, FLJ43543, MGC75043, Small cell lung carcinoma cluster 4 antigen |
Formulation | PBS |
Gene ID (Entrez) | 100133941 |
Classification | Monoclonal |
Immunogen | The immunogen was the full length version of the protein: CD24 (ML5) |
Primary or Secondary | Primary |
Clone | ML5 |
RBFOX3/NeuN Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody has been used in 2 publications
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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Target Species | Human,Mouse,Rat |
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Western Blot,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen) |
Form | Antisera |
Isotype | IgG |
Gene Accession No. | A6NFN3 |
Research Discipline | DNA replication Transcription Translation and Splicing, Neuronal Cell Markers, Neuroscience |
Concentration | 1 mg/ml |
Antigen | RBFOX3/NeuN |
Gene Symbols | RBFOX3 |
Regulatory Status | RUO |
Purification Method | Affinity purified |
Dilution | Western Blot 1:5000-1:10000, Simple Western 1:500, Immunohistochemistry 1:500-1:1000, Immunocytochemistry/Immunofluorescence 1:500-1:1000, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating 1:500-1:1000 |
Molecular Weight of Antigen | 48 kDa |
Gene Alias | FLJ56884, FLJ58356, Fox-1 homolog C, FOX3, FOX-3, hexaribonucleotide binding protein 3, HRNBP3, NeuN, neuronal nuclei, RBFOX3, RNA binding protein fox-1 homolog 3, RNA binding protein, fox-1 homolog (C. elegans) 3 |
Gene ID (Entrez) | 146713 |
Formulation | 50% PBS, 50% glycerol with 5mM Sodium Azide |
Immunogen | The N-terminal 100 amino acids of human RBFOX3/NeuN as expressed in and purified from E. coli. [UniProt# A6NFN3] |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | Human, bovine, porcine, mouse, rat and other mammals. |
NeuN Mouse anti-Avian, Chicken, Ferret, Human, Mouse, Pig, Rat, Salamander, Clone: A60, MilliporeSigma™
Mouse Monoclonal Antibody
KAP1, p Ser824 Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody has been used in 31 publication
p19ARF/CDKN2A Antibody (12-A1-1), Novus Biologicals™
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Rat Monoclonal Antibody has been used in 5 publications
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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Target Species | Mouse,Hamster (Negative),Human (Negative) |
Host Species | Rat |
Conjugate | Unconjugated |
Applications | Western Blot,ELISA,Immunohistochemistry,Immunocytochemistry,Immunofluorescence,Immunoprecipitation,Immunohistochemistry (Paraffin) |
Form | Ascites |
Isotype | IgG2b |
Gene Accession No. | Q64364 |
Research Discipline | Cancer, Cell Biology, Cell Cycle and Replication, Chromatin Research, p53 Pathway, Transcription Factors and Regulators, Tumor Suppressors |
Antigen | p19ARF |
Gene Symbols | CDKN2A |
Regulatory Status | RUO |
Purification Method | Unpurified |
Dilution | Western Blot 1:500, ELISA 1:5000-1:10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500 |
Molecular Weight of Antigen | 19 kDa |
Gene Alias | ARF, CDK4 inhibitor p16-INK4, CDK4IP14ARF, CDKN2, cell cycle negative regulator beta, CMM2P16-INK4A, Cyclin-dependent kinase 4 inhibitor A, cyclin-dependent kinase inhibitor 2A, cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4), INK4, INK4A, MLMP16INK4, MTS-1, MTS1P14, Multiple tumor suppressor 1, p14, p14ARF, p16, p16-INK4, p16INK4a, p16-INK4a, P19, p19ARF, TP16 |
Gene ID (Entrez) | 12578 |
Formulation | Ascites with 0.1% Sodium Azide |
Immunogen | A synthetic peptide made to a sequence (within residues 1-100) of mouse p19ARF. [UniProt# Q64364] |
Classification | Monoclonal |
Primary or Secondary | Primary |
Test Specificity | This antibody is specific for murine p19ARF. It does not recognize human p14Arf nor Golden hamster p13Arf. |
Clone | 12-A1-1 |
Target Species | Chicken,Human,Mouse,Non-human Primate,Rat,Zebrafish |
---|---|
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Flow Cytometry,Immunocytochemistry,Immunofluorescence,Immunohistochemistry,Western Blot |
Gene Accession No. | O14556, P00356, P04406, P04797, P16858, Q5MJ86, Q5XJ10, Q64467, Q9ESV6 |
Isotype | IgG |
Antigen | GAPDH |
Gene Symbols | GAPDH, GAPDHS |
Gene Alias | BARS-38; bb02e05; BOS_5957; cb609; CDABP0047; EC 1.2.1.12; G3PD; G3PDH; Gapd; gapdh; gapdh.L; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; HGNC:4141; I79_001391; KNC-NDS6; mg:bb02e05; MGC88685; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; wu:fb33a10; wu:ft80f05; XELAEV_18036725mg |
Gene | GAPDH |
Gene ID (Entrez) | 14433, 14447, 24383, 2597, 26330, 317743, 374193, 406367, 66020 |
Formulation | TBS with 1% BSA, 40% Glycerol and 0.05% sodium azide; pH 7.4 |
Immunogen | Human GAPDH aa 112-333. |
Classification | Monoclonal |
Clone | SA30-1 |
Nestin Antibody, Novus Biologicals™
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Chicken Polyclonal Antibody has been used in 46 publications
Content And Storage | Store at 4C in the dark. |
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Host Species | Chicken |
Conjugate | Unconjugated |
Isotype | IgY |
Research Discipline | Stem Cell Markers |
Antigen | Nestin |
Gene Symbols | NES |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Gene Alias | FLJ21841, nestin |
Gene ID (Entrez) | 10763 |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | Nestin |
LC3B Antibody (1251D) - BSA Free, Novus Biologicals™
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Rabbit Monoclonal Antibody has been used in 1 publication
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
---|---|
Target Species | Human,Mouse,Rat |
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Western Blot,Immunocytochemistry,Immunofluorescence,Flow Cytometry,KnockDown |
Form | Purified |
Isotype | IgG |
Research Discipline | Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience |
Concentration | 1.0 mg/mL |
Antigen | LC3B |
Gene Symbols | MAP1LC3B |
Regulatory Status | RUO |
Purification Method | Protein A or G purified |
Dilution | Western Blot 1 - 2 ug/ml, Simple Western 5 ug/ml, Immunocytochemistry/Immunofluorescence 5 ug/ml, Flow (Intracellular) 1 ug/ml, Knockout Validated |
Molecular Weight of Antigen | 14.688 kDa |
Gene Alias | anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B monoclonal, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B |
Gene ID (Entrez) | 81631 |
Formulation | PBS with 0.02% Sodium Azide |
Immunogen | Recombinant monoclonal LC3B Antibody (1251D) was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [Uniprot: Q9GZQ8]. |
Classification | Monoclonal |
Primary or Secondary | Primary |
Clone | 1251D |