Growth Factor Products
Growth Factor Products
Fibroblast Growth Supplement 5 ml
Greater than 95% by SDS-PAGE analysis. Stemfactor bFGF is shipped at room temperature. Lyophilized bFGF is stable for up to 6 months from date of receipt when stored at −20 °C to −80 °C. Reconstituted bFGF, at concentrations greater than or equal to 0.1 mg/mL, is stable for up to 3 months when stored at −20 °C and up to 6 months when stored at −80 °C.
NERVE GROWTH FACT MOUSE 100UG
Purified Mouse Nerve Growth Factor 2.5s
Use stem cell factor (SCF) to promote cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. SCF is an early-acting cytokine that activates PI3K, JAK/STAT, and MAPK pathways upon binding to its receptor. Together with other cytokines like thrombopoietin and Flt3/Flk-2 Ligand, SCF is used to promote expansion of primitive hematopoietic stem cells and multipotent progenitor cells in culture (Martin et al. Cell, 1990; Kent et al. Clin Cancer Res, 2008.). In combination with various growth factors and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy VC. Blood, 1997). Human recombinant SCF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 15 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor.
Use platelet-derived growth factor (PDGF) AA to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells (Piao et al. Cell Stem Cell, 2015). PDGF is a dimeric glycoprotein that signals through the receptor tyrosine kinases PDGFRα and PDGFRβ. It is a potent mitogen for cells of mesenchymal origin and induces migration through signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al. Growth Factors, 2007). PDGF-AA mediates proliferation of oligodendrocyte progenitor cells and oligodendrocyte lineage differentiation through the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) (Hu et al. Neuroscience, 2008). Human recombinant PDGF-AA from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 50 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: GDGF, Glioma-derived growth factor, ODGF, Osteosarcoma-derived growth factor, Platelet-derived growth factor-AA.
Akron’s Human Serum Albumin (HSA) 25% Solution is manufactured, tested, and released following relevant cGMP guidelines for blood-derived ancillary materials and supported by a Type II Master File (MF) on file with the FDA which can be referenced during your drug or biologic application process. Akron’s HSA 25% Solution is specifically formulated for cell and gene therapy commercial manufacturing.
Increases the stimulation of B cells. Alternative name: CD154, TNFSF 5, gp39. MW: ~18kDa (SDS-PAGE). Concentration: 0.1mg/ml after reconstitution. Endotoxin content: E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG-tag. Specificity: Binds to human CD40. Use/Stability: Stable for at least 6 months after receipt when stored at -20°C. UniProt: P29965. Application: ELISA. Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. Long Term Storage: -20°C
Vascular endothelial growth factor (VEGF-165) is a heparin-binding homodimeric glycoprotein involved in embryonic vasculogenesis and angiogenesis. VEGF binds to VEGFR-1 (R1) and VEGFR-2 (R2), and activates Raf/MEK/ERK and PI3K/AKT pathways (Ferrara et al.). It plays an important role in neurogenesis both in vitro and in vivo (Storkebaum et al.). It has neurotrophic effects on neurons of the central nervous system and promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara et al.).
Basic fibroblast growth factor (bFGF) is a prototypic member of the fibroblast growth factor family. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman & Klagsbrun; Kimelman & Kirschner) and are involved in a variety of biological processes including cell proliferation, differentiation, survival, and apoptosis (Folkman & Klagsbrun; Klagsbrun; Rifkin & Moscatelli). bFGF has the β-trefoil structure (Ponting & Russell), binds to the four FGF receptor (FGFR) family members, and activates JAK/STAT, PI3K, ERK1/2, and other receptor tyrosine kinase (RTK) signaling pathways. It supports the maintenance of undifferentiated human embryonic stem cells (Xu et al.; Kang et al.), stimulates human embryonic stem cells to form neural rosettes (Zhang et al.), and improves proliferation of human mesenchymal stem cells and enhances chondrogenic differentiation (Solchaga et al.).
Epidermal growth factor (EGF) is characterized by high affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter & Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). Central nervous system stem cells also proliferate in response to the EGF stimulus (Reynolds & Weiss).
InVitria’s Optiferrin recombinant human transferrin is completely animal- and blood-free. Its functional performance in cell culture is equivalent to human holo-transferrin in terms of reversible iron binding, transferrin-receptor binding, and promoting cell growth and productivity. It is compatible with a variety of cell types and applications including stem, primary, bioproduction cells, proliferation of human HSC, and Sp2/0 hybridoma cells. Optiferrin offers significant regulatory advantages due to its safety and consistency.Made in the USA.
Use macrophage colony-stimulating factor (M-CSF) to regulate the proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a homodimeric glycoprotein growth factor that is crucial to the development of tissue-resident macrophages in most tissues (Ginhoux & Jung Nat Rev Immunol, 2014) and regulates inflammatory responses together with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al. Immunity, 2014). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) (Hamilton JA J Leukoc Biol, 1997). Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam & Ghosh Tumour Biol, 2014). Mouse recombinant M-CSF from STEMCELL comes lyophilized with ≥ 95% purity; EC50 ≤ 10 ng/mL; endotoxin levels verified ≤ 1 EU/μg protein. Alternative names: Colony stimulating factor 1, CSF-1.
Fibroblast growth factor 7 (FGF-7) is a member of the FGF family, and acts exclusively through a subset of FGF receptor isoforms expressed predominantly by epithelial cells (Finch & Rubin). FGF-7 seems to act specifically on epithelial cells and stimulates proliferation, migration, and differentiation of these cells, and also participates in epithelial protection and repair both in vitro and in vivo (Finch & Rubin; Werner). In contrast, FGF-7 is produced solely by cells of mesenchymal origin, and functions as a paracrine mediator of mesenchymal-epithelial communication (Rubin et al.). FGF-7 has also been shown to supplement several wound-healing properties of bioengineered skin (Erdag et al.) and to induce autophagy in human keratinocytes (Belleudi et al.). Additionally, FGF-7 has a role in pluripotent stem cell differentiation to endodermal pancreatic-like insulin-producing cells and thymic epithelial cells (Inami et al.; Niu et al.).