Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.

1 – 15 of 139 results

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
Compatibility	qPCR Cyclers
For Use With (Application)	Gene Expression,Genotyping,siRNA Validation
PCR Method	qPCR
Product Line	ABsolute Blue
Detection Method	SYBR

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	qPCR Master Mix
For Use With (Equipment)	7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Luminaris
Detection Method	SYBR

Thermo Scientific™ DyNAmo HS SYBR Green qPCR Kit

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Tbr DNA polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Kit Type	DyNAmo HS SYBR Green qPCR Kit
Concentration	2X (DyNAmo HS SYBR Green qPCR Mix), 50X (ROX Dye)
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	DyNAmo
Detection Method	SYBR

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Promotions are available

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Purity or Quality Grade	LD (Low DNA)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	SYBR™

Thermo Scientific™ ABsolute Blue QPCR Mix, ROX

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Format	Tube
Product Type	qPCR Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
PCR Method	qPCR
Product Line	ABsolute Blue
Detection Method	Primer-probe

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
Compatibility	qPCR Cyclers
For Use With (Application)	Gene Expression,Genotyping,siRNA Validation
PCR Method	qPCR
Product Line	ABsolute
Detection Method	SYBR

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Applied Biosystems™ TaqMan™ Universal PCR Master Mix is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

Promotions are available

Pricing & Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Promotions are available

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	PowerUP™, SYBR™
Detection Method	SYBR

Promega GoTaq™ Probe qPCR Master Mix

GoTaq™ Probe qPCR Master Mix is a ready-to-use stabilized 2X formulation containing GoTaq™ Hot Start Polymerase, MgCl2, dNTPs and proprietary reaction buffer.

Pricing & Availability
Specifications

Concentration	2X
Compatibility	Both standard and fast instrument cycling programs
For Use With (Application)	Gene Expression

Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

Thermo Scientific™ ABsolute QPCR Mix, ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	qPCR Mix
For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	ABsolute
Detection Method	Primer-probe

Thermo Scientific™ DyNAmo Flash SYBR Green qPCR Kit

Developed for fast real-time qPCR, the kit provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Tbr DNA polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type	DNA, dsDNA
Reaction Speed	Fast
Kit Type	DyNAmo Flash SYBR Green qPCR Kit
Concentration	2X (DyNAmo Flash SYBR Green qPCR Mix), 50X (ROX Dye)
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	DyNAmo Flash
Detection Method	SYBR

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Product Type	qPCR Master Mix
For Use With (Equipment)	7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Luminaris
Detection Method	SYBR

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	Maxima
Detection Method	SYBR

Thermo Scientific™ ABsolute Blue QPCR Mix, low ROX

ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments.

Promotions are available

Pricing & Availability
Specifications

Polymerase	Taq DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Format	Tube
Product Type	qPCR Mix
For Use With (Equipment)	7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type	DNA, dsDNA
Reaction Speed	Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	ABsolute Blue
Detection Method	Primer-probe

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Real Time PCR Master Mixes

Real Time PCR Master Mixes

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