Real Time PCR Master Mixes

Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
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1 – 30 of 135 results
1

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, no ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR
2

Thermo Scientific™ ABsolute Blue QPCR Mix, with separate ROX vial

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Format Tube
Product Type qPCR Mix
For Use With (Equipment) BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
PCR Method qPCR
Product Line ABsolute Blue
Detection Method Primer-Probe
3

Thermo Scientific™ ABsolute Blue QPCR Mix, low ROX

ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Format Tube
Product Type qPCR Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute Blue
Detection Method Primer-Probe
4

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, low ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
5

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
6

Thermo Scientific™ ABsolute QPCR Mix, ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method Primer-Probe
7

Thermo Scientific™ DyNAmo Flash SYBR Green qPCR Kit

Developed for fast real-time qPCR, the kit provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance.

Polymerase Tbr DNA polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Fast
Kit Type DyNAmo Flash SYBR Green qPCR Kit
Concentration 2X (DyNAmo Flash SYBR Green qPCR Mix), 50X (ROX Dye)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line DyNAmo Flash
Detection Method SYBR
8

Thermo Scientific™ DyNAmo HS SYBR Green qPCR Kit

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

Polymerase Tbr DNA polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Standard
Kit Type DyNAmo HS SYBR Green qPCR Kit
Concentration 2X (DyNAmo HS SYBR Green qPCR Mix), 50X (ROX Dye)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line DyNAmo
Detection Method SYBR
9

Thermo Scientific™ DyNAmo ColorFlash SYBR Green qPCR Kit

Offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting.

Polymerase Tbr DNA polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
Sample Type DNA, dsDNA
Reaction Speed Fast
Kit Type DyNAmo ColorFlash SYBR Green qPCR Kit
Concentration 2X (DyNAmo ColorFlash SYBR Green qPCR), 50X (ROX Dye), 40X (Sample Buffer Solution with Yellow Dye)
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line DyNAmo Flash
Detection Method SYBR
10

Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
11

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
12

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
13

Thermo Scientific™ Maxima SYBR Green/Fluorescein qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
14

Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X)

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Maxima
Detection Method SYBR
15

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, low ROX

Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
16

Thermo Scientific™ Luminaris Color HiGreen qPCR Master Mix, high ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
17

Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, low ROX

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Luminaris
Detection Method SYBR
18

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, low ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR
19

Thermo Scientific™ ABsolute QPCR Mix, low ROX

Optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type qPCR Mix
For Use With (Equipment) 7500 System, QuantStudio™, ViiA™ 7 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line ABsolute
Detection Method Primer-Probe
20

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility Block-Based qPCR Instruments
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute Blue
Detection Method SYBR
21

Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
Compatibility qPCR Cyclers
For Use With (Application) Gene Expression,Genotyping,siRNA Validation
PCR Method qPCR
Product Line ABsolute
Detection Method SYBR
22

Thermo Scientific™ ABsolute Blue QPCR Mix, ROX

ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity.

Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Format Tube
Product Type qPCR Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Sample Type DNA, dsDNA
Reaction Speed Standard
Concentration 2X
PCR Method qPCR
Product Line ABsolute Blue
Detection Method Primer-Probe
23

Promega GoTaq™ Probe qPCR Master Mix

GoTaq™ Probe qPCR Master Mix is a ready-to-use stabilized 2X formulation containing GoTaq™ Hot Start Polymerase, MgCl2, dNTPs and proprietary reaction buffer.

Concentration 2X
Compatibility Both standard and fast instrument cycling programs
For Use With (Application) Gene Expression
24

Invitrogen™ Platinum™ SYBR™ Green qPCR SuperMix-UDG w/ROX

Ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.

ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum™, SYBR™
Detection Method SYBR
25

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX

qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.

ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum™
Detection Method Primer-Probe
26

Invitrogen™ SYBR GreenER™ qPCR SuperMix for iCycler™ Instrument

Incorporate the latest high-performance technology to provide the most reliable gene expression data from your iCycler iQ™ or MyiQ™ instrument.

ENCOMPASS_PREFERRED
Polymerase Taq DNA Polymerase
GC-Rich PCR Performance High
Content And Storage 2X SYBR™ GreenER™ qPCR SuperMix for iCycler™ instrument contains: hot-start Taq DNA polymerase,
SYBR™ GreenER™ fluorescent dye, fluorescein, MgCl2, dNTPs (with dUTP instead of dTTP), UDG, and stabilizers.

Store at 4°C upon receipt. Guaranteed stable for 6 months when properly stored.
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum™, SYBR™ GreenER™
Detection Method SYBR
27

Invitrogen™ EXPRESS qPCR Supermix, universal

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.

ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Format Tube
Product Type Express qPCR Supermix
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-Probe
28

Invitrogen™ EXPRESS qPCR Supermix, with premixed ROX

EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.

ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Content And Storage EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format Tube
Product Type Express qPCR Supermix
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method Primer-Probe
29

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

ENCOMPASS_PREFERRED
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-Probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan™
30

Applied Biosystems™ TaqMan™ GTXpress™ Master Mix

Combines AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format.

Promotions are available
ENCOMPASS_PREFERRED
GC-Rich PCR Performance High
Concentration 2X
For Use With (Application) Genotyping
PCR Method qPCR