PCR Equipment and Supplies
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PCR Equipment and Supplies
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Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
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GC-Rich PCR Performance | High |
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Format | 1-step RT-PCR Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 13 kb |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Hot Start | Built-in Hot Start |
Optimal Reaction Temperature | 50°C to 65°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
PCR Method | 1-step RT-PCR |
Multiplex Capability | Yes |
Detection Method | Gel Electrophoresis |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
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GC-Rich PCR Performance | High |
---|---|
Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 12.3 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV RT Reaction Buffer
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase.
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Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
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GC-Rich PCR Performance | High |
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Format | Master Mix |
Final Product Type | First-Strand cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Concentration | 4X |
Technique | Template Switching Reverse Transcription |
Starting Material | Cells or RNA |
Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
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Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Green Features | Less Hazardous, Less Waste |
For Use With (Application) | RT-PCR,Real Time PCR (qPCR) |
Format | Kit |
Reaction Format | Premixed Components |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | Cells |
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum SuperFi |
Format | Kit |
Size (Final Product) | Up to 14 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 45°C to 60°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents
The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.
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Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).
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Shipping Condition | Dry Ice |
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Polymerase | Platinum SuperFi U DNA Polymerase |
GC-Rich PCR Performance | High |
Format | Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 10 kb |
Final Product Type | PCR Amplified cDNA |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription, Preamplification |
Starting Material | Cells or RNA |
For Use With (Application) | Next-Generation Sequencing (NGS),Real Time PCR (qPCR) |