Life Science Buffers
![](https://assets.fishersci.com/TFS-Assets/CCG/product-images/life-science-buffers-header-image.jpg-250.jpg)
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (1)
- (84)
- (1)
- (4)
- (1)
- (81)
- (1)
- (1)
- (1)
- (1)
- (3)
- (1)
- (10)
- (15)
- (1)
- (1)
- (2)
- (1)
- (114)
- (101)
- (1)
- (3)
- (8)
- (2)
- (5)
- (16)
- (21)
- (17)
- (1)
- (4)
- (1)
- (8)
- (53)
- (19)
- (5)
- (35)
- (130)
- (2)
- (1)
- (23)
- (2)
- (1)
- (1)
- (1)
- (1)
- (12)
- (2)
- (1)
- (27)
- (1)
- (7)
- (5)
- (9)
- (83)
- (107)
- (4)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (4)
- (115)
- (3)
- (3)
- (1)
- (3)
- (3)
- (7)
- (5)
- (3)
- (6)
- (1)
- (472)
- (5)
- (4)
- (4)
- (94)
- (28)
- (11)
- (23)
- (1)
- (4)
- (2)
- (129)
- (1)
- (11)
- (3)
- (14)
- (49)
- (2)
- (34)
- (2)
- (13)
- (29)
- (2)
- (10)
- (9)
- (9)
- (23)
- (46)
- (6)
- (4)
- (42)
- (8)
- (5)
- (14)
- (2)
- (3)
- (2)
- (3)
- (1)
- (9)
- (1)
- (96)
- (3)
- (5)
- (4)
- (3)
- (5)
- (3)
- (10)
- (3)
- (31)
- (4)
- (5)
- (9)
- (3)
- (45)
- (1)
- (3)
- (1)
- (4)
- (1)
- (1)
- (2)
- (1)
- (3)
- (5)
- (1)
- (5)
- (3)
- (5)
- (1)
- (6)
- (5)
- (5)
- (3)
- (2)
- (1)
- (10)
- (1)
- (2)
- (3)
- (2)
- (2)
- (2)
- (5)
- (7)
- (2)
- (4)
- (2)
- (2)
- (1)
- (3)
- (3)
- (3)
- (1)
- (2)
- (1)
- (3)
- (3)
- (3)
- (15)
- (4)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (1)
- (4)
- (2)
- (4)
- (3)
- (10)
- (14)
- (39)
- (2)
- (2)
- (8)
- (3)
- (1)
- (36)
- (3)
- (3)
- (5)
- (32)
- (14)
- (39)
- (1)
- (9)
- (2)
- (1)
- (1)
- (9)
- (1)
- (42)
- (1)
- (1)
- (7)
- (1)
- (1)
- (2)
- (1)
- (2)
- (2)
- (1)
- (18)
- (5)
- (3)
- (1)
- (17)
- (3)
- (5)
- (4)
- (6)
- (85)
- (96)
- (3)
- (6)
- (12)
- (72)
- (1)
- (40)
- (6)
- (3)
- (20)
- (1)
- (10)
- (4)
- (1)
- (5)
- (12)
- (2)
Filtered Search Results
![](/catalog/search/static/resources/images/icons/back_to_top_icon.png)
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
![BIOPHARMA](/content/dam/fishersci/glyphs/sure_trace.png)
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
With low endotoxin content.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Dilution | 1 X |
---|---|
Product Type | DPBS |
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Corning™ Cell Culture Phosphate Buffered Saline (1X)
With low endotoxin content. Without calcium and magnesium.
Dilution | 1 X |
---|---|
Content And Storage | 2° to 30°C |
Form | Liquid |
Formulation | Potassium Dihydrogen Phosphate 0.144g/L, Sodium Chloride 9g/L, Disodium Phosphate 0.795g/L |
pH | 7.3 to 7.5 |
Boiling Point | 100°C |
---|---|
Name Note | 1X Solution, pH 7.4 |
CAS | 77-86-1 |
Protease | Protease free |
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
Synonym | TBS |
DNase | DNase free |
Recommended Storage | RT |
ChemAlert Storage Symbol | Gray |
Gibco™ PBS, pH 7.4
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
![GREENER_CHOICE](/content/dam/fishersci/glyphs/greener_choice.png)
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Room Temperature |
---|---|
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Dilution | 1 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ DPBS, calcium, magnesium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
![GREENER_CHOICE](/content/dam/fishersci/glyphs/greener_choice.png)
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Dilution | 1 X |
---|---|
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.2 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Corning™ Molecular Biology Reagents
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
![BIOPHARMA](/content/dam/fishersci/glyphs/sure_trace.png)
Supports traceability with guaranteed access to certificates and proactive change notifications.
Learn More
Used in molecular biology, nucleic acid purification applications
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Dilution | 10 X |
---|---|
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Color | Colorless |
pH | 7.4 |
Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Formulation | 50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide |
---|---|
Physical Form | Liquid |
pH | 7.7 |
Chemical Name or Material | Running Buffer |
Recommended Storage | Contents: NuPAGE™ MOPS SDS Running Buffer (20X) Storage: +4°C to 25°C |
Concentration | 20X |
Product Line | NuPAGE |
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 8.0 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
Gibco™ PBS (10X), pH 7.4
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
![GREENER_CHOICE](/content/dam/fishersci/glyphs/greener_choice.png)
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
Form | Liquid |
Color | White |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2800 - 3100 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 77-86-1,7732-18-5,60-00-4,10043-35-3 Synonym: TBE
![EDGE](/content/dam/fishersci/glyphs/edge.png)
CAS | 77-86-1,7732-18-5,60-00-4,10043-35-3 |
---|---|
Synonym | TBE |
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
![GREENER_CHOICE](/content/dam/fishersci/glyphs/greener_choice.png)
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2630 - 3000 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 6.7 - 7.0 |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ DPBS, no calcium, no magnesium
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
![GREENER_CHOICE](/content/dam/fishersci/glyphs/greener_choice.png)
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
![ENCOMPASS_PREFERRED](/content/dam/fishersci/glyphs/encompass_preferred.png)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
Learn More
Form | Liquid |
---|---|
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.3 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 270 - 300 mOsm/kg |
Solution Type | Dulbecco's Phosphate Buffered Saline |