Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Content And Storage | Room temperature |
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Form | Liquid |
Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
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Supplier Diversity Partner
Small and/or diverse supplier based on Federal laws and SBA requirements.
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Supports traceability with guaranteed access to certificates and proactive change notifications.
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Small and/or diverse supplier based on Federal laws and SBA requirements.
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With low endotoxin content
Dilution | 1 X |
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Product Type | DPBS |
Boiling Point | 100°C |
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Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Gibco™ ACK Lysing Buffer
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Formulation | 50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide |
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Physical Form | Liquid |
pH | 7.7 |
Chemical Name or Material | Running Buffer |
Recommended Storage | Contents: NuPAGE™ MOPS SDS Running Buffer (20X) Storage: +4°C to 25°C |
Concentration | 20X |
Product Line | NuPAGE |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)
Made with high-purity reagents and are strictly quality controlled
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Formulation | 1X: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3 |
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Physical Form | Liquid |
pH | 7.3 |
Chemical Name or Material | Running Buffer |
Concentration | 20X |
Product Line | NuPAGE |
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
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Dilution | 10 X |
Content And Storage | Store at room temperature. |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.4 |
Product Line | Ambion™ |
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
Color | Undesignated |
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Physical Form | Liquid |
Chemical Name or Material | HEPES Buffer |
Grade | Molecular Biology |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
Concentration | 0.95 to 1.05 M |
ChemAlert Storage Symbol | Gray |
Absorbance | ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm |
Name Note | 1M Solution, pH 7.3 |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.2 to 7.5 |
Synonym | N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Formula | C8H18N2O4S |
Formula Weight | 238.197 |
Color | Colorless |
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Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 8.0 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 7732-18-5,60-00-4,10043-35-3,77-86-1 Synonym: TBE
CAS | 7732-18-5,60-00-4,10043-35-3,77-86-1 |
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Synonym | TBE |
Color | Undesignated |
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Physical Form | Liquid |
Chemical Name or Material | Tris-Glycine-SDS |
Grade | Electrophoresis |
Identification | Pass Test |
DNase | DNase free |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS. |
CAS | 151-21-3 |
Protease | Protease free |
Synonym | TG-SDS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
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Physical Form | Liquid |
pH | 8 |
Grade | Molecular Biology |
Recommended Storage | Store at room temperature. |
Concentration | 1 M |
Product Line | Ambion |
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Chemical Name or Material | Running Buffer |
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Concentration | 10X |
Product Line | Novex |