Cytiva Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Supplier:  Cytiva 27084601

Catalog No. 45-001-320

Description

• Supplied as lyophilized powder
• Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
• Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
• Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

Specifications

• Buffer: Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na₂HPO₄, 1.8mM KH₂PO₄ pH 7.3
• Product Type: Thrombin Protease Fusion Protein
• Quantity: 500U
• For Use With (Application): Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours